Two sediment depth cores were collected from a mud sediment patch at the sea floor of the Irish Sea. Cores were collected by the University of Manchester. Cores were sliced at 1 cm intervals from 0 - 10 cm, and at 2 cm intervals thereafter. Slicing was performed in an anaerobic bag. Samples were transferred to Newcastle University for DNA extraction. A total of 21 samples were extracted for core 1, and 23 samples extracted for core 2.

Soil depth core collected from the Needle’s Eye site in Dumfries, Scotland. Clear plastic depth core was lowered into a bog within the site, excised, and capped at the top and bottom. Core was sliced at 1 cm intervals at the University of Manchester in an anaerobic bag. A total of 41 samples were generated. Soil samples were returned to Newcastle University.

Two sediment depth cores were collected from the floor of Loch Etive, near Oban, Scotland. Slicing was performed in an anaerobic bag. Samples were taken at 0.5 cm increment between 0 - 2 cm, 1 cm increments between 2 - 10 cm, and at either 2 or 5 cm increments thereafter. Samples were transferred to Newcastle University for DNA extraction. A total of 21 samples were extracted for core 1, and 23 samples extracted for core 2.

Data from laboratory experiments conducted as part of project NE/K011464/1 (associated with NE/K011626/1) Multiscale Impacts of Cyanobacterial Crusts on Landscape stability. Soils were collected from two sites in eastern Australia and transferred to a laboratory at Griffith University, Queensland for conduct of experiments. Soils were A, a sandy loam, and B a loamy fine sand. Trays 120 mm x 1200 mm x 50 mm were filled with untreated soil that contained a natural population of biota. Soils were either used immediately for experiments (physical soil crust only: PC) or were placed in a greenhouse and spray irrigated until a cyanobacterial crust has grown from the natural biota. Growth was for a period of 5 days (SS), c.30 days (MS2) or c.60 days (MS1). Following the growing period (if applicable) trays were placed in a temperature/humidity controlled room at 35º and 30% humidity until soil moisture (measured 5 mm below the surface) was 5%. Trays were then subject to rainfall simulation. Rainfall intensity of 60 mm hr-1 was used and rainfall was applied for 2 minutes (achieving 2 mm application), 8 minutes (achieving 8 mm application) or 15 minutes (achieving 15 mm application). Following rainfall, trays were returned to the temperature/humidity-controlled room under UV lighting until soil moisture at 5 mm below the surface was 5%. A wind tunnel was then placed on top of each tray in turn and a sequential series of wind velocities (5, 7, 8.5, 10, 12 m s-1) applied each for one minute duration. On each tray the five wind velocities were run without saltation providing a cumulative dust flux. For the highest wind speed, an additional simulation run was conducted with the injection of saltation sands. Three replicates of each rainfall treatment were made. Variables measured include photographs, spectral reflectance, surface roughness, fluorescence, penetrometry, chlorophyll content, extracellular polysaccharide content, Carbon, Nitrogen and splash erosion and particle-size analysis (of wind eroded material). Details of rainfall simulator, growth of cyanobacteria, laser soil surface roughness characterisation and wind tunnel design and deployment in Strong et al., 2016; Bullard et al. 2018, 2019. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018a. Impact of multi-day rainfall events on surface roughness and physical crusting of very fine soils. Geoderma, 313, 181-192. doi: 10.1016/j.geoderma.2017.10.038. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018b. Effects of cyanobacterial soil crusts on surface roughness and splash erosion. Journal of Geophysical Research – Biogeosciences. doi: 10.1029/2018. Strong, C.S., Leys, J.F., Raupach, M.R., Bullard, J.E., Aubault, H.A., Butler, H.J., McTainsh, G.H. 2016. Development and testing of a micro wind tunnel for on-site wind erosion simulations. Environmental Fluid Mechanics, 16, 1065-1083.

The <250um fraction of 28 household vacuum dust samples were extracted using high throughput isolation of microbial genomic DNA (21 samples from a national campaign within the UK and 7 samples from Greece, providing samples from two contrasting bioclimatic zones). Both positive and negative reagent controls were included to ensure sterility throughout the processing and sequencing steps, and a randomly selected sample was run in triplicate (DSUK179). These data (raw fastq files: Target_gene 16S and Target_subfragment V4) are available from the European Nucleotide Archive via the study accession PRJEB46920 with individual sample accession numbers ERX6130460 to ERX6130493; https://www.ebi.ac.uk/ena/browser/view/PRJEB46920). A wide range of anthropogenic factors are likely to affect the indoor microbiome and to capture some of this heterogeneity participants were asked to complete a questionnaire. In addition, trace element data were generated using an X-Ray fluorescence spectrometry on the <250um sieved fraction of the household vacuum dust. Sample location data are provided at town/city, Country level. Indoor dust serves as a reservoir for environmental exposure to microbial communities, many of which are benign, some are beneficial, whilst some exhibit pathogenicity. Whilst non-occupational exposure to a range of trace elements and organic contaminants in house dust are a known risk factor for a range of diseases and poor health outcomes, we know far less about the microbial communities associated with our indoor home environments, and their interaction/impacts on human health. Our knowledge of indoor residential bacterial biodiversity, biogeography and their associated drivers are still poorly understood. The data were collected to improve our understanding of the home microbiome.

Data from laboratory experiments conducted as part of project NE/K011464/1 (associated with NE/K011626/1) Multiscale Impacts of Cyanobacterial Crusts on Landscape stability. Soils were collected from two sites in eastern Australia and transferred to a laboratory at Griffith University, Queensland for conduct of experiments. Soils were A, a sandy loam, and B a loamy fine sand. Trays 120 mm x 1200 mm x 50 mm were filled with untreated soil that contained a natural population of biota. Soils were either used immediately for experiments (physical soil crust only: PC) or were placed in a greenhouse and spray irrigated until a cyanobacterial crust has grown from the natural biota. Growth was for a period of 5 days (SS), c.30 days (MS2) or c.60 days (MS1). Following the growing period (if applicable) trays were placed in a temperature/humidity controlled room at 35° and 30% humidity until soil moisture (measured 5 mm below the surface) was 5%. Trays were then subject to rainfall simulation. Rainfall intensity of 60 mm hr-1 was used and rainfall was applied for 2 minutes (achieving 2 mm application), 8 minutes (achieving 8 mm application) or 15 minutes (achieving 15 mm application). Following rainfall, trays were returned to the temperature/humidity-controlled room under UV lighting until soil moisture at 5 mm below the surface was 5%. A wind tunnel was then placed on top of each tray in turn and a sequential series of wind velocities (5, 7, 8.5, 10, 12 m s-1) applied each for one minute duration. On each tray the five wind velocities were run without saltation providing a cumulative dust flux. For the highest wind speed, an additional simulation run was conducted with the injection of saltation sands. Three replicates of each rainfall treatment were made. Variables measured include photographs, spectral reflectance, surface roughness, fluorescence, penetrometry, chlorophyll content, extracellular polysaccharide content, Carbon, Nitrogen and splash erosion and particle-size analysis (of wind eroded material). Details of rainfall simulator, growth of cyanobacteria, laser soil surface roughness characterisation and wind tunnel design and deployment in Strong et al., 2016; Bullard et al. 2018, 2019. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018a. Impact of multi-day rainfall events on surface roughness and physical crusting of very fine soils. Geoderma, 313, 181-192. doi: 10.1016/j.geoderma.2017.10.038. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018b. Effects of cyanobacterial soil crusts on surface roughness and splash erosion. Journal of Geophysical Research – Biogeosciences. Doi: 10.1029/2018. Strong, C.S., Leys, J.F., Raupach, M.R., Bullard, J.E., Aubault, H.A., Butler, H.J., McTainsh, G.H. 2016. Development and testing of a micro wind tunnel for on-site wind erosion simulations. Environmental Fluid Mechanics, 16, 1065-1083.

Data from laboratory experiments conducted as part of project NE/K011464/1 (associated with NE/K011626/1) Multiscale Impacts of Cyanobacterial Crusts on Landscape stability. Soils were collected from two sites in eastern Australia and transferred to a laboratory at Griffith University, Queensland for conduct of experiments. Soils were A, a sandy loam, and B a loamy fine sand. Trays 120 mm x 1200 mm x 50 mm were filled with untreated soil that contained a natural population of biota. Soils were either used immediately for experiments (physical soil crust only: PC) or were placed in a greenhouse and spray irrigated until a cyanobacterial crust has grown from the natural biota. Growth was for a period of 5 days (SS), c.30 days (MS2) or c.60 days (MS1). Following the growing period (if applicable) trays were placed in a temperature/humidity controlled room at 35° and 30% humidity until soil moisture (measured 5 mm below the surface) was 5%. Trays were then subject to rainfall simulation. Rainfall intensity of 60 mm hr-1 was used and rainfall was applied for 2 minutes (achieving 2 mm application), 8 minutes (achieving 8 mm application) or 15 minutes (achieving 15 mm application). Following rainfall, trays were returned to the temperature/humidity-controlled room under UV lighting until soil moisture at 5 mm below the surface was 5%. A wind tunnel was then placed on top of each tray in turn and a sequential series of wind velocities (5, 7, 8.5, 10, 12 m s-1) applied each for one minute duration. On each tray the five wind velocities were run without saltation providing a cumulative dust flux. For the highest wind speed, an additional simulation run was conducted with the injection of saltation sands. Three replicates of each rainfall treatment were made. Variables measured include photographs, spectral reflectance, surface roughness, fluorescence, penetrometry, chlorophyll content, extracellular polysaccharide content, Carbon, Nitrogen and splash erosion and particle-size analysis (of wind eroded material). Details of rainfall simulator, growth of cyanobacteria, laser soil surface roughness characterisation and wind tunnel design and deployment in Strong et al., 2016; Bullard et al. 2018, 2019. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018a. Impact of multi-day rainfall events on surface roughness and physical crusting of very fine soils. Geoderma, 313, 181-192. doi: 10.1016/j.geoderma.2017.10.038. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018b. Effects of cyanobacterial soil crusts on surface roughness and splash erosion. Journal of Geophysical Research – Biogeosciences. doi: 10.1029/2018. Strong, C.S., Leys, J.F., Raupach, M.R., Bullard, J.E., Aubault, H.A., Butler, H.J., McTainsh, G.H. 2016. Development and testing of a micro wind tunnel for on-site wind erosion simulations. Environmental Fluid Mechanics, 16, 1065-1083.

This dataset presents field measurements of the biological response of cyanobacterial soil crusts to rainfall and of the impact of this response on the susceptibility of the soil surface to wind erosion. The data are in Excel spreadsheets and record cyanobacteria fluorescence, the presence of chlorophyll a and exocellular polysaccharide, soil surface strength, particle size distribution and soil loss by wind erosion. The study was located within Diamantina National Park (23°36’44.8”S; 143°17’46.9”E) in the north-eastern part of the Lake Eyre basin, central Australia. Site characteristics are 1/A physical depositional crust; 2/B cyanobacterial crust on dune flank; 3/D cyanobacterial crust on claypan; 4/E physical structural crust; 5/C cyanobacterial crust on nebkha field. Different amounts of rainfall were applied using Griffith University Mobile Rainfall Simulator (see Bullard et al. 2018 for technical details). Following rainfall and drying in situ of the surface, wind erosion was measured using a portable mini-wind tunnel (see Strong et al. 2016 for technical details). The data will be of value for understanding cyanobacterial response to different rainfall amounts and wind speeds under future climate scenarios. The project principal investigator was Prof. Joanna Bullard and data Quality Assurance by Dr. Helene Aubault. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018. Impact of multi-day rainfall events onsurface roughness and physical crusting of very fine soils. Geoderma, 313, 181-192. doi: 10.1016/j.geoderma.2017.10.038. Strong, C.L., Leys, J.F., Raupach, M.R., Bullard, J.E., Aubault, H.A., Butler, H.J., McTainsh, G.H. 2016. Development and testing of a micro-wind tunnel for on-site wind erosion simulations. Environmental Fluid Mechanics 16, 1065-1083.

Data from laboratory experiments conducted as part of project NE/K011464/1 (associated with NE/K011626/1) Multiscale Impacts of Cyanobacterial Crusts on Landscape stability. Soils were collected from two sites in eastern Australia and transferred to a laboratory at Griffith University, Queensland for conduct of experiments. Soils were A, a sandy loam, and B a loamy fine sand. Trays 120 mm x 1200 mm x 50 mm were filled with untreated soil that contained a natural population of biota. Soils were either used immediately for experiments (physical soil crust only: PC) or were placed in a greenhouse and spray irrigated until a cyanobacterial crust has grown from the natural biota. Growth was for a period of 5 days (SS), c.30 days (MS2) or c.60 days (MS1). Following the growing period (if applicable) trays were placed in a temperature/humidity controlled room at 35° and 30% humidity until soil moisture (measured 5 mm below the surface) was 5%. Trays were then subject to rainfall simulation. Rainfall intensity of 60 mm hr-1 was used and rainfall was applied for 2 minutes (achieving 2 mm application), 8 minutes (achieving 8 mm application) or 15 minutes (achieving 15 mm application). Following rainfall, trays were returned to the temperature/humidity-controlled room under UV lighting until soil moisture at 5 mm below the surface was 5%. A wind tunnel was then placed on top of each tray in turn and a sequential series of wind velocities (5, 7, 8.5, 10, 12 m s-1) applied each for one minute duration. On each tray the five wind velocities were run without saltation providing a cumulative dust flux. For the highest wind speed, an additional simulation run was conducted with the injection of saltation sands. Three replicates of each rainfall treatment were made. Variables measured include photographs, spectral reflectance, surface roughness, fluorescence, penetrometry, chlorophyll content, extracellular polysaccharide content, Carbon, Nitrogen and splash erosion and particle-size analysis (of wind eroded material). Details of rainfall simulator, growth of cyanobacteria, laser soil surface roughness characterisation and wind tunnel design and deployment in Strong et al., 2016; Bullard et al. 2018, 2019. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018a. Impact of multi-day rainfall events on surface roughness and physical crusting of very fine soils. Geoderma, 313, 181-192. doi: 10.1016/j.geoderma.2017.10.038. Bullard, J.E., Ockelford, A., Strong, C.L., Aubault, H. 2018b. Effects of cyanobacterial soil crusts on surface roughness and splash erosion. Journal of Geophysical Research – Biogeosciences. doi: 10.1029/2018. Strong, C.S., Leys, J.F., Raupach, M.R., Bullard, J.E., Aubault, H.A., Butler, H.J., McTainsh, G.H. 2016. Development and testing of a micro wind tunnel for on-site wind erosion simulations. Environmental Fluid Mechanics, 16, 1065-1083.

Soil depth core collected from the Needle’s Eye site in Dumfries, Scotland. Clear plastic depth core was lowered into a bog within the site, excised, and capped at the top and bottom. Core was sliced at 1 cm intervals at the University of Manchester in an anaerobic bag. A total of 42 samples were generated. Soil samples were returned to Newcastle University.