Date (Creation)

2020-02-21

Identifier

http://data.bgs.ac.uk/id/dataHolding/13607593

Maintenance and update frequency

notApplicable notApplicable

GEMET - INSPIRE themes, version 1.0

• Geology

BGS Thesaurus of Geosciences

• Bacteria
• Uranium
• NGDC Deposited Data

dataCentre

• NGDC Deposited Data

Keywords

• NERC_DDC

Access constraints

otherRestrictions Other restrictions

Other constraints

licenceOGL

Other constraints

Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Use constraints

otherRestrictions Other restrictions

Other constraints

The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints

Available under the Open Government Licence subject to the following acknowledgement accompanying the reproduced NERC materials "Contains NERC materials ©NERC [year]"

Metadata language

EnglishEnglish

Topic category

• Geoscientific information

Geographic identifier

Dumfries and Galloway [id=31903]

Date (Revision)

2009

Begin date

2014-12-02

End date

2014-12-02

Distribution format

• FASTQ file ()

OnLine resource

Data

Hierarchy level

dataset Dataset

Other

dataset

Statement

DNA was extracted using FastDNA SPIN Kit for Soil. PCR using the 515F and 926R primers targeting the V4-V5 region of the 16S rRNA gene. Amplicon length was 481 bp. PCRs were 25 uL reactions with 22 uL of MegaMix Blue, 1uL of forward and 1 uL of reverse primer at 100 pM concentration, and 1 uL of 1:10 diluted template DNA. Initial denaturation was 95C for 5 minutes, followed by 30 cycles of 95C for 1 minute, 55C for 1 minutes, 72C for 1 minute, and a final elongation step of 72C for 10 minutes. Each depth sample was run in triplicate and pooled. Pooled amplicons were cleaned using Agencourt AMPure XP. PCR amplicons were quantified via Qubit 3.0 fluorometer and a total dilution factor was calculated for each sample, diluting each sample to 1000. Equimolar samples were pooled into a final library. Sequencing was performed on the Ion Torrent PGM on a 316 chip.

File identifier

a0043087-00a3-2ad1-e054-002128a47908 XML

Metadata language

EnglishEnglish

Hierarchy level

dataset Dataset

Date stamp

2025-05-03

Metadata standard name

UK GEMINI

Metadata standard version

2.3

Dataset URI

http://data.bgs.ac.uk/id/dataHolding/13607593