The database contains fasta sequences from UniProt and associated metadata for molluscan shell matrix proteins (SMPs). The database only contains SMPs that have been experimentally validated to be present in molluscan shell matrices (based on the publication(s) attached to the UniProtID). Metadata includes information on functional domains present in the sequence, as detected by InterproScan. With the advent of Next Generation Sequencing technologies, it is computationally resource intensive to run sequence similarity algorithms on all published data. Moreover, it is impractical to sort through hundreds of sequence similarity search results when working with non-model organisms, since pre-established functional annotations of sequences are generally not available. Therefore, this database was created in order to provide a targeted molluscan biomineralization dataset for sequence similarity algorithms (such as BLAST).
Temperature depth devices (TDR) were attached to northern rockhopper penguins breeding on Nightingale (37 deg 25''S, 12 deg 28''W) to investigate temporal differences in diving behaviour throughout the species'' breeding cycle. The data sets comprise the unprocessed pressure and temperature data for each bird in .csv format. Data were collected as part of the Darwin Plus funded Project Pinnamin, 2016 - 2018.
Geolocators (GLS) were attached to northern rockhopper penguins breeding on Nightingale Island (37 deg 25 S, 12 deg 28 W: 4 km2) with the aim to study the species'' dispersal in the inter-breeding period, 2017. This data successfully augmented and complement existing data sets for the species breeding on Nightingale Island and enhanced our understanding of the species'' distribution at sea throughout their annual cycle. There are four different data types for each penguin a) the unprocessed light sensor data, b) the unprocessed activity (wet/dry) data, c) the unprocessed temperature data and d) the estimated positions using the BAStag and SGAT packages in R. Data were collected as part of the Darwin Plus funded Project Pinnamin, 2016 - 2018.
Genetic variation on a spatial scale was assessed, using both DNA fingerprinting and sequencing-based approaches, in the Antarctic endemics Buellia frigida, Carbonia vorticosa and Amandinea petermananii, and in the bipolar species Caloplaca saxicola, Umbilicaria decussata and Cladonia galindezii. PCR-based (Polymerase Chain Reaction) molecular biology techniques, were used as they are ideal for working with lichens because little starting material is required. See Fabian et al. 2007 for further information on analyses and results.
The majority of Antarctic lichens produce sexual organs, and in many species sexual ascospores appear to be the only reproductive propagule. However, it is unknown whether sexual reproduction involves selfing (homothallism) or outcrossing (heterothallism). To investigate this issue we have established axenic cultures of sexual progeny in order to generate DNA fingerprints and thereby determine the breeding system.
The fieldwork involved collection of fertile lichens from a range of sites across the Antarctic Peninsula and isolation of the lichen-forming fungi into pure culture in a laboratory at Rothera. Approximately 5,600 monospore cultures were isolated, including B frigida. Approximately 400 thalli of Usnea species, and 3 O. frigida thalli have also been collected for whole thallus analysis. Logarithmic sampling transects of B frigida were conducted at Rothera (2 transects) and on Anchorage Island (one transect) to examine the genetic variation and geographic variation. All thalli of B frigida collected from the transects were successfully used to generate viable spores from four individual apothecia from each thallus. 16 spores were subcultured and maintained from each apothecium.
Adelie and Chinstrap penguins were fitted with a combined GPS and time-depth recorder (TDR) tags for between two and fourteen days in order to log their three-dimensional foraging trips. Tags were deployed between December and February of 2008 with a total of 19 Adelie penguin tracks and 35 Chinstrap penguin tracks.
The chronology of Gentoo penguins on Bird Island, South Georgia has been monitored since 1988 (no data for 1989-90 or 1990-91). 30 nests are marked in the colony at Square Pond, and 40 in the Johnson Beach colony. Regular checks are made to record when eggs are laid and the peak laying date (when 75% of nests have eggs) is calculated.
The weights of fledging Macaroni penguin (<i>Eudyptes chrysolophus</i>) chicks at Bird Island have been monitored since 1989. The colony at Fairy Point, also known as Little Mac, contains approximately 500 breeding pairs. Each year, just before their departure from the colony, a sample of 100 fledgling birds are weighed as part of the Bird Island long-term monitoring programme. This data is submitted to the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) as part of their Ecosystem Monitoring Programme (CEMP).
The total number of seals on Signy Island is counted in February each year. The island is divided into 6 areas, sub-divided into 105 zones and the number of Antarctic Fur seals, Elephant seals, Weddell seals and other seal species in each zone is counted typically over a two day period. For Fur seals the number of pups, white seals and entangled seals are also recorded. The first recorded count was conducted in 1974 and counts have been done annually since 1985.