Non -contact atomic force microscopy (NC-AFM) images of surface nanobubbles on the fluorcarbonate mineral synchysite. Synchysite is a rare earth fluorcarbonate mineral which has previously been relatively unstudied. Since nanobubbles were first imaged in 2000, they have been thought to play a intigral role in mineral processing. Images of nanobubbles were produced under collector reagent conditions favourable to flotation. These are the first images of nanobubbles on the fluorcarbonate mineral synchysite. Nanobubbles at the surface of synchysite improve the understanding of both flotation and nanobubble formation.

Modal mineralogy data of iceberg-rafted debris deposited at IODP Site U1538 in the Scotia Sea 1.2 million years ago based on QEMSCAN® analyses, which infer minerals from chemistry

These data show images recorded using a variety of methods of a model system of bacterial metal reduction. In all cases the bacteria grew from a pure culture of Geobacter sulfurreducens, and grew undisturbed on thin films of amorphous Fe oxyhydroxide – ferrihydrite. The different imaging methodologies have highlighted different features of this interaction. AFM shows the surface texture of the bacteria and ferrihydrite films; epifluorescence was used to allow counting of the cells at different time points from 0 to 12 days post inoculation (cell counts available in excel spreadsheet); and confocal imaging allow visualisation of the redox patterns surrounding cells and to identify areas of bioreduced Fe(II) (quantification of Fe(II) available in excel spreadsheet). The following data is included: 1. 9 x AFM images of Geobacter sulfurreducens bacteria growing on ferrihydrite films 2. 5 x epifluorescence images of Geobacter sulfurreducens bacteria growing on ferrihydrite films over time 3. spreadsheet bacterial counts associated with epifluorescence images 4. 7 x confocal images of Geobacter sulfurreducens bacteria growing on ferrihydrite films with redox green staining of appendages 5. 5 x example confocal images of Geobacter sulfurreducens bacteria growing on ferrihydrite films with Fe(II) highlighted by RhoNox-1 6. Spreadsheet of quanitfication of RhoNox intensity against bacteria and Fe co-location Data is presented which shows the formation of precious metal nanoparticles on the surface of geobacter sulfurreducens cells. The images were produced by CryoTEM. Full details of the experiment are available in this publication http://onlinelibrary.wiley.com/doi/10.1002/ppsc.201600073/full 7. Powerpoint presentation of TEM images of precious metal nanoparticles formed on the surface of Geobacter cells