Biota lipid concentrations
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The impacts of Persistent Organic Pollutants (POPs) are assessed in fat tissue (blubber) using live explant samples from young grey seals (Halichoerus grypus) on the east coast of Scotland. The data show POP levels in blubber and responses to intrinsic pollutants and experimental exposure to polychlorinated biphenyls (PCBs), relevant due to its applicability to other species, including humans. The study sites are located on the Isle of May (56° 10’ 57” N, 02° 33’ 17” W) between October and December 2015, 2016, and 2017, where the grey seal population had been monitored annually and from where previous POP data from blubber tissue was investigated in 2002. A second sample set was collected from yearling grey seals hauled out on the Isle of May, Moray Firth, and Culbin Sands (57° 39’10” N, 03° 43’ 15” W) and maintained in temporary captivity at the Sea Mammal Research Unit between February and August 2018. The capture and explant data includes animal identifier, moulting state (where relevant for yearlings), whether the animal was fed or fasted (where relevant for pups), body and sample tissue mass. Characteristics of the animals like sex, birth, and weaning date (where known), rate of mass change, life history stage and blubber fat content, including intrinsic blubber POP levels, were measured at the Centre for Analytical Research and Technology at the University of Liège, Belgium to be used as covariates in a Generalised Additive Mixed Model (GAMM). This analysis was undertaken after accelerated solvent extraction of the samples in hexane dichloromethane (Dionex 200 by Thermo Fisher Scientific) and the extracts were analysed using a gas chromatograph (Agilent 6890 GC) coupled to Autospec Ultima High-Res Mass Spectrometer. The response of explants was tested through measurement of gene expression (using the Stratagene Mx3000P qPCR System or StepOne Plus Real-Time PCR System), insulin signaling and glucose uptake, lactate production, oxygen consumption and lipolytic rate using Randox Monza assays. Thyroid hormones triiodothyronine (TT3) and thyroxine (TT4) were measured using Enzyme Linked Immunosorbent Assays (ELISA Fortress Diagnostics Ltd). The live explants were treated overnight with a mix of PCBs and organochlorine pesticide derivatives (DDT, DDE), which were associated with altered metabolic function symptoms such as glucose uptake and lactate production in 2015-2017. The data will facilitate an understanding of the molecular mechanisms of contaminant-induced disruption of energy balance by legacy pollutants in seals during their most vulnerable life stages. The data collection was led by Dr Kimberley Bennett from Abertay University and funded by the NERC Discovery Science project “Obesogens in a naturally obese animal: An experimental approach to assess the impact of marine pollutants on fat tissue function in seals”, parent grant reference NE/M013723/1 with child grant NE/M01357X/1.
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The Marine Environment Monitoring and Assessment National database (MERMAN) is a national database which holds and provides access to data collected under the Clean Safe Seas Environmental Monitoring Programme (CSEMP) formerly the National Marine Monitoring Programme (NMMP). The data collected are the responsibility of the Competent Monitoring Authorities (CMAs) who collect the samples from stations in UK waters using water sampling techniques, trawls, nets or grabs. The CMAs then send the collected samples to accredited laboratories where they are analysed. A weighting is calculated, based on the quality of the analysis. The weighting score incorporates the laboratory accreditation, reference material, inter-laboratory comparisons, detection limits, uncertainties and standard deviations. Where data do not meet a threshold score they are given a status of ‘FAIL’ and although they are stored they are not made available to external users. The contaminants and biological effects in biota data start in 1987 with greater use of the database occurring from 1997 onwards. Data are submitted by the CMAs annually and an annual submission may include updates to legacy data to provide additional data or improve data/metadata. The data held in MERMAN fulfils the UK's mandatory monitoring requirements under the Oslo and Paris Convention (OSPAR) Joint Assessments and Monitoring Programme (JAMP). These data are used in support of European Commission (EC) directives and national assessments, such as Charting Progress 2 and are also supplied to the European Marine Observation and Data Network (EMODNET).
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The Marine Environment Monitoring and Assessment National database (MERMAN) is a national database which holds and provides access to data collected under the Clean Safe Seas Environmental Monitoring Programme (CSEMP) formerly the National Marine Monitoring Programme (NMMP). The data collected are the responsibility of the Competent Monitoring Authorities (CMAs) who collect the samples from stations in UK waters using water sampling techniques, trawls, nets or grabs. The CMAs then send the collected samples to accredited laboratories where they are analysed. A weighting is calculated, based on the quality of the analysis. The weighting score incorporates the laboratory accreditation, reference material, inter-laboratory comparisons, detection limits, uncertainties and standard deviations. Where data do not meet a threshold score they are given a status of ‘FAIL’ and although they are stored they are not made available to external users. The MERMAN dataset starts in 1987 with greater use of the database occurring from 1997 onwards. Data are submitted by the CMAs annually and an annual submission may include updates to legacy data to provide additional data or improve data/metadata. The data held in MERMAN fulfils the UK's mandatory monitoring requirements under the Oslo and Paris Convention (OSPAR) Joint Assessments and Monitoring Programme (JAMP). These data are used in support of European Commission (EC) directives and national assessments, such as Charting Progress 2 and are also supplied to the European Marine Observation and Data Network (EMODNET).
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The dataset contains hydrographic, biogeochemical and biological measurements of ocean and seabed sediment properties. Hydrographic profiles provided measurements of temperature, salinity, dissolved oxygen and fluorescence to accompany biogeochemical and biological samples, including concentrations of nutrients, particulate organic carbon (POC) and particulate organic nitrogen (PON). Emphasis was placed on the collection of benthic data, with numerous core samples being collected and analysed for pigments, biomarkers, lipids and other organic compounds. Samples were also collected and analysed for Holothurian species, while a large volume in situ filtration system was used to measure biogeochemical variables including POC and PON, and particulate iron. Station data were supplemented by continuous underway measurements of bathymetry, current velocities, sea surface salinity, temperature, fluorescence and beam attenuation across the survey area. These were accompanied by underway measurements of surface meteorological parameters including irradiance, air temperature, humidity, sea level pressure and wind velocities. The data were collected across the Crozet Plateau in the Southern Indian Ocean between 1st December 2005 and 14th January 2006 on RRS Discovery cruise D300. Data collection focused on four sites, with repeated hydrographic profiles, water and sediment samples collected at each location. In total, 89 instrumentation deployments were carried out at the four stations, including a conductivity-temperature-depth (CTD) package and a Megacorer (for sediment sampling). The underway system utilised a hull-mounted acoustic Doppler current profiler (ADCP) as well as a thermosalinograph and other standard surface hydrographic and meteorological instruments. Further data were collected using a variety of equipment including an otter trawl (net) and submersible cameras but these data are currently held by the data originators and are undergoing processing so are not included in the parameter and instrument lists above. The principal objective of Benthic CROZEX was to assess the manner in which biogeochemical composition and flux of organic matter to the deep-sea floor drives benthic community structure, dynamics and diversity at sites with contrasting primary production regimes. Investigators from British Antarctic Survey (BAS), Natural History Museum (NHM), National Oceanographic Centre (NOC) and the National University of Ireland, Galway (NUIG) were involved. Data management is being undertaken by the British Oceanographic Data Centre (BODC) but data processing is ongoing and various data are yet to be submitted to BODC.
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The Marine Productivity (MarProd) programme data set comprises physical, biological and biogeochemical data, including hydrographic profiles (temperature, salinity, fluorescence, dissolved oxygen, photosynthetically active radiation (PAR)), and samples of nutrients, suspended particulate material, dissolved material, phytoplankton and zooplankton. These data were supplemented by continuous underway measurements of bathymetry, surface hydrography (temperature, salinity, fluorescence and attenuance), meteorology (wind speed and direction, PAR and total incident irradiance, air temperature, humidity and atmospheric pressure), remote sensing of sea surface temperature and ocean colour, and by production experiments. The data were collected in UK shelf seas and the North Atlantic Ocean between 2000 and 2002. Surface biogeochemical parameters (nutrients, photosynthesis, production, suspended particulate material and dissolved organic material) were measured during cruises in the Irish Sea in May, June and July 2001, while ongoing hydrographic and plankton time-series at Plymouth Marine Laboratory's L4 sampling station are also included in the data set. However, the bulk of the data were collected during a major fieldwork campaign of four dedicated research cruises on RRS Discovery between November 2001 and December 2002. A total of over 800 gear deployments were performed at 159 stations mainly distributed in the Irminger Sea and Iceland Basin, facilitating the measurement of water mass distribution, velocity field and mixed layer properties. A comprehensive water sampling programme was undertaken for the purposes of plant pigment and microscopic analyses; biomass estimations of different taxonomic/functional groups of microplankton (picoplankton, phytoplankton and microzooplankton); high resolution profiles of inorganic nutrient concentrations; and determination of abundance of key zooplankton species (Calanus finmarchicus, Oithona spp. and euphausiids). Process studies were undertaken to obtain information about factors controlling the reproduction, growth, mortality and behaviour of individual species using physiological studies (feeding experiments, egg production and nauplii development, species interactions) and analyses of biochemical composition (lipids and hormones studies, analyses of carbon/nitrogen and stable isotope ratios composition). Phytoplankton primary production was measured using carbon uptake on the last two cruises and additional data were collected using a Fast Repetition Rate Fluorometer (FRRF) through continuous surface underway sampling and vertical deployments. MarProd's main objective was to investigate the population dynamics of key zooplankton species in UK shelf seas and in the northern Atlantic with emphasis on the manner in which physical factors such as water temperature and oceanic currents influence their distribution, abundance and productivity. The MarProd Programme involved researchers from numerous institutions from the UK, Ireland and Spain. The data are archived at the British Oceanographic Data Centre.
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The UK Surface Ocean-Lower Atmosphere Study (UK SOLAS) marine fieldwork data set comprises all data, marine or otherwise, collected during sea-going activities. The fieldwork included eight dedicated research cruises in the eastern North Atlantic Ocean, spanning the period 2006-2008. These cross-disciplinary missions resulted in a diverse data catalogue. This includes meteorology (3-D wind speed and direction, total irradiance, Photosynthetically Active Radiation/PAR, air temperature, atmospheric pressure, humidity, aerosol optical thickness); atmospheric composition (carbon dioxide concentration, aerosol particle counts and size spectra, chemical analyses of aerosol particle composition, cloud condensation nuclei/CCN, concentrations of pollutants such as black carbon, concentrations of free radical species such as iodine monoxide and nitrate radicals); chemical and energy-fluxes across the air-sea boundary (dust deposition rates, oxygen and nitrogen fluxes, carbon dioxide fluxes, sensible heat fluxes, latent heat fluxes, momentum fluxes); biological, chemical and physical properties and processes in the sea surface micro-layer (chlorophyll concentration, bacterial production, phytoplankton and bacterial speciation, concentrations of biogenic trace compounds such as halocarbons, nitrous oxide, dimethyl sulphide/DMS and alcohols, surfactant concentrations, halogen concentrations such as iodine, iodide and iodate); biological, chemical and photochemical properties and processes in the ocean subsurface (primary productivity, trace gas production, plankton community composition, nutrient concentration, concentrations of trace metals such as iron, aluminium, manganese, magnesium and cobalt, ligand and complex metal chemistry parameters such as heme, dust dissolution, salinity, temperature, amino acids and urea, carbonate system chemistry including alkalinity); and sea-state physics (breaking waves, wave slope, whitecaps, bubble size spectra, aerosol formation, subsurface acoustics). Additionally, time series of air-sea fluxes were measured from the Norwegian weather ship, Polarfront, between 2006 and 2009. UK SOLAS scientists also participated in the Bergen Mesocosm experiment during 2008. This simulated gas exchanges and biological, chemical and photochemical properties and processes in the sea surface micro-layer under controlled conditions. The study united atmospheric and marine scientists from institutions across the UK and international collaborators. The UK SOLAS data set was intended to advance understanding of the mutual interactions between the atmosphere and the oceans, especially the chemical exchanges that affect ocean productivity, atmospheric composition and climate. It was funded by the UK Natural Environment Research Council, as the UK's contribution to the international Surface Ocean-Lower Atmosphere Study (SOLAS). The data are held at the British Oceanographic Data Centre (BODC) and have been incorporated into the National Oceanographic Database (NODB). Data collected from non-ship based activities, for example land-based atmospheric data and data resulting from campaigns using the Facility for Airborne Atmospheric Measurements (FAAM) aircraft are held at the British Atmospheric Data Centre (BADC).