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This is a dataset obtained from analysis of lake sediment and overlying water from six sites along a depth gradient, in Loch Leven, Scotland, over a period of one year. Parameters measured from the water and included in the dataset are dissolved oxygen concentration, conductivity, pH, temperature, concentrations of three forms of phosphorus, and ammonium and silica concentrations. Chlorophyll a concentration measured from the sediment surface is included, and within the sediment concentrations of seven different forms of phosphorus are provided. Full details about this dataset can be found at https://doi.org/10.5285/76a2bd9a-fb02-4f37-91b9-4835eb31ab7b