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This research sought to explore the pattern of population movement (direction, rate, permanency) along a hypothesised route from Africa to Australasia during Oxygen Isotope Stage 4. Using GIS-based analyses and hypothetical models of population movement, potential routes out of East Africa were generated and examined. The goal of these analyses was to assess the viability of particular routes, and consider them in terms of ecological and geographical constraints. As a result, several routes through Africa, Arabia, South Asia, Southeast Asia, and Australasia were proposed and evaluated. These routes have been further examined with regards to archaeological site location, the timing of human presence in South Asia, and biological indicators of human diversity.