This dataset contains fitness-linked life history traits, environmental data, and protein and gene expression data for Daphnia magna. Life history data were measured in common garden experiments. For each clone of the same genotype across all experiments, size at maturity (distance between the head and the base of the tail spine), age at maturity (first time eggs were observed in the brood chamber), fecundity (total number of offspring released summing first and second brood), and mortality were measured. Critical thermal maximum (CTmax) was measured on the experimental animals. Environmental data consisted of Secchi disk depth (water transparency) ; total phosphorous; and total nitrogen for the years 1971–1999; a record of pesticides 1955–2010; temperature records over the past century 80 km from Lake Ring. Organic and carbonate contents of the sediment was estimated using the loss on ignition (LOI) method. Hb protein data consisted of constitutive Hb protein crude content animals reared in normoxic (saturated oxygen level) conditions at two experimental temperatures, 20 and 30°C. Heat shock protein expression was measured in four heat shock proteins (HSP20, HSP60, HSP70 and HSP90). Total RNA, qPCR, mean CT (cycle threshold) value per sample and per protein were collected. Full details about this dataset can be found at https://doi.org/10.5285/13f878dc-ef87-45d5-be8c-5733c88c30e9

The data contain metrics of risk to earthworms, springtails, lacewings, parasitic wasps, bumblebees, honeybees and two solitary bees (Osmia bicornis and Osmia cornuta) from exposure to pesticides applied to arable crops in 5 Km grid cells in England every other year from 1994 to 2016. The metrics were derived from pesticide application rates and toxicity metrics from exotoxicology experiments. The data was derived to investigate spatial and temporal patterns of risk from exposure to pesticides for non-target invertebrates. Full details about this dataset can be found at https://doi.org/10.5285/9406c9e9-7b41-4f15-84be-dbbf98fe372e

These maps show the Input to Yield Ratio (IYR) of wheat farming in England, calculated as the ratio of agrochemical inputs to agricultural reward as yield. We assess the IYR at a 1km resolution for wheat grown in England over the period the period 2010-2017, considering the different agrochemicals used and their differing forms of environmental impact. We provide four mapped products: IYR for Nitrogen fertilisers, IYR for Phosphorus fertilisers, IYR for pesticide risk to earthworms, and IYR for pesticide risk to honeybees. The IYR is calculated using scaled rather than absolute values, with the maximum value in each yield and inputs dataset set as 1, so that all inputs and rewards are expressed proportionally to each other. The research was funded under research programme NE/N018125/1 ASSIST and NE/W005050/1 AgZero+. Full details about this dataset can be found at https://doi.org/10.5285/dfe2a4a5-2b3a-4731-ba7f-aea7e926f1dd

Triazine, organophosphate and pyrethroid (TOP) pesticide data from river water collected at various sites within the Humber catchment area as part of the Land Ocean Interaction Study project (LOIS).. Water samples were collected weekly and during storm periods between 1994 and 1996. The unfiltered samples were extracted on the day of collection using solid-phase-extraction (SPE), the SPE cartridges stored overnight at 4 degreesC and then transported to the Institute of Freshwater Ecology (IFE), Wareham for elution, drying and Gas Chromatography (GC) analysis. Pesticides measured were Atrazine, Cyanazin, Desmetryn, Fenitrothion, HCH gamma, Malathion, Parathion, Permethrin, Prometryn, Simazine, Terbutryn. The sampling and extraction of the pesticides was carried out by the LOIS team at York University and the elution and sample preparation for GC analysis was done at IFE. Full details about this dataset can be found at https://doi.org/10.5285/b6af91ff-fc56-4ec2-81b8-d384eedcc343

Data from analyses of addled and deserted sea eagle eggs collected by licensed collectors in Scotland. Contaminants reported include polychlorinated biphenyls (PCBs), organochlorine pesticides or their persistent metabolites, and a range of metals and metaloids. The white-tailed sea eagle has been re-introduced to a number of Scottish Islands since the 1980s. The Predatory Bird Monitoring Scheme (PBMS) is a long-term, national monitoring scheme that quantifies the concentrations of contaminants in the livers and eggs of selected species of predatory and fish-eating birds in Britain. Full details about this dataset can be found at https://doi.org/10.5285/72ed6237-aedf-43a7-b9e3-eef95320a2bb

[THIS DATASET HAS BEEN WITHDRAWN]. This data product maps pesticide applications across England and Wales. It is produced at a 1km resolution with units of kg active ingredient applied per year. Pesticide application rates (kg/km2/yr) are calculated for each of the crops grown in each 1km square, with the total application calculated by multiplying the estimated rates by the area of each crop in the square. The product provides application estimates for 129 different active ingredients including herbicides, insecticides, molluscicides and fungicides. Uncertainty maps are produced alongside each active ingredient map to quantify the level of confidence in the estimated applications. Uncertainty is quantified using the distribution of each parameter estimate obtained from the modelling method and is expressed relative to the total application. The product is a snapshot of average applications between 2012 and 2016. The product builds upon the Centre for Ecology & Hydrology (CEH) Land Cover® Plus: Crops product. An average of CEH Land Cover® Plus: Crops 2015, 2016 and 2017 is used to reflect average crop coverage at the 1km resolution. Temporal variation in pesticide application is not modelled explicitly but is reflected in the uncertainty maps. This data product was funded by the Natural Environment Research Council (NERC) under research programme NE/N018125/1 Achieving Sustainable Agricultural Systems (ASSIST). ASSIST is an initiative jointly supported by NERC and the Biotechnology and Biological Sciences Research Council (BBSRC). Full details about this dataset can be found at https://doi.org/10.5285/a72f8ce8-561f-4f3a-8866-5da620c0c9fe

CEH Land Cover plus: Pesticides maps annual average pesticide applications across England, Wales and Scotland. The product provides application estimates for 162 different active ingredients including herbicides, insecticides, molluscicides and fungicides. It is produced at a 1km resolution with units of kg active ingredient applied per year, averaged between 2012 and 2017. Pesticide application rates (kg/km2/yr) are calculated for each of the crops grown in each 1km square, using information from CEH Land Cover® Plus: Crops 2015, 2016 and 2017 to determine where each crop is grown. Pesticide application data is provided by the Pesticide Usage Survey. Uncertainty maps are produced alongside each active ingredient map to quantify the level of confidence in the estimated applications. Uncertainty is quantified using the distribution of each parameter estimate obtained from the modelling method and is expressed relative to the total application. The product builds upon the Centre for Ecology & Hydrology (CEH) Land Cover® Plus: Crops product. These maps were created under the NERC funded ASSIST (Achieving Sustainable Agricultural Systems) project to enable exploration of the impacts of agrochemical usage on the environment, enabling farmers and policymakers to implement better, more sustainable agricultural practices. Full details about this dataset can be found at https://doi.org/10.5285/99a2d3a8-1c7d-421e-ac9f-87a2c37bda62