soil bacteria
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This dataset contains information on the performance of soil eubacteria inoculated into soil microcosms and exposed to a series of experimental droughts (or control conditions) in an experimental evolution study. Isolates of Bacillus and Pseudomonas included in the experimental evolution study had been sampled from the roots of Festuca ovina in the control treatment at the Buxton Climate Change Impacts Lab (BCCIL). Variables measured included estimates of microbial population size (via qPCR), microcosm soil respiration in response to substrate addition (in a substrate utilisation profiling assay) and water loss from the soil in response to the experimental drought/control treatments. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
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This dataset contains information on the performance of soil microbial communities (from field soil washes), soil synthetic microbial populations (from isolates within a single bacterial genus) and plants each originating from either drought and control long-term climate treatments at the Buxton Climate Change Impacts Lab (BCCIL), in a microcosm experiment. Plant and microbe provenance were manipulated to effect different combinations of climate ancestry in different microcosm pots. In addition, a factorial watering treatment (control or drought treatment) was applied. Isolates of Pseudomonas used to found experimental populations of soil microbes had been sampled from the roots of Festuca ovina in the control and drought treatments at BCCIL. Variables measured included soil respiration, plant traits, plant available soil nutrients, soil enzyme activities, soil respiration responses to substrate addition (in a substrate utilisation profiling assay), and soil respiration responses to drought (in a substrate utilisation profiling assay). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/e09db3ad-c54a-4f99-bf3c-37ee63fc2998
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Data on CO2 and CH4 exchange rates between soil and atmosphere, soil temperatures, bacterial 16S ribosomal RNA genes, fungal internal transcribed spacer 2 (ITS2) copies and the relative abundances of the 40 most abundant bacterial taxa in the 48 plots of a soil warming and irrigation experiment on Svalbard in the High Arctic. On 2014-09-10, a soil warming and irrigation experiment was set up at Kongsfjordneset on the Brogger Peninsula, Svalbard. Warming was applied continuously with open top chambers and the irrigation treatment was applied in mid-late June and late August each year. Greenhouse gas exchange between the soil and atmosphere was measured on 2018-08-23 and 2018-08-26. At this time, soil samples were taken for DNA analyses and the amount of bacterial and fungal DNA present in soil was measured. The 40 most frequent bacterial operational taxonomic units were also determined. This project was funded by UK Natural Environment Research Council (core funding to the British Antarctic Survey), the Danish National Research Foundation (CENPERM DNRF100) and Seoul National University.
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