NERC Data Catalogue Service
Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms
This dataset contains information on the performance of soil eubacteria inoculated into soil microcosms and exposed to a series of experimental droughts (or control conditions) in an experimental evolution study. Isolates of Bacillus and Pseudomonas included in the experimental evolution study had been sampled from the roots of Festuca ovina in the control treatment at the Buxton Climate Change Impacts Lab (BCCIL). Variables measured included estimates of microbial population size (via qPCR), microcosm soil respiration in response to substrate addition (in a substrate utilisation profiling assay) and water loss from the soil in response to the experimental drought/control treatments. Full details about this nonGeographicDataset can be found at
https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
Simple
- Date (Publication)
- 2024-05-13
- Date (Creation)
- 2023-03-10
- Identifier
- doi: / 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
- Other citation details
- Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions Other restrictions
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
-
- Biota
- Environment
- Distribution format
-
-
Comma-separated values (CSV)
()
-
Comma-separated values (CSV)
()
- OnLine resource
-
Download the data
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- OnLine resource
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Supporting information
Supporting information available to assist in re-use of this dataset
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Other
- nonGeographicDataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- Bacterial isolates from BCCIL (twelve from each of genera Bacillus and Pseudomonas) were raised in liquid media and used to inoculate sterilised soil in kilner jars (approx 100 ml soil in 500 ml jars). Each isolate of each bacterial taxon was inoculated into five replicate microcosms per moisture treatment (drought and control) and microcosms were assigned random positions within four experimental blocks. Microcosms in the control treatment were topped up weekly to a volumetric water content of 40%. Those in the drought treatment were subjected to a series of four simulated droughts lasting six weeks, where watering was not carried out. Soil moisture content determination and watering was carried out by assessing the mass of each kilner jar and the dried soil it initially contained, allowing controlled watering to the target soil water content. Substrate utilisation profiling was carried out by taking soil samples from the microcosms at the end of the final drought cycle, which were amended with different substrates (water, glucose, cellulose, artificial root exudate)in MicroResp(TM) colourimetric microplates, before CO2 efflux detection on a FLUOstar Omega spectrophotometer (BMG Labtech, Ortenberg, Germany). q-PCR was carried out by taking soil samples from the microcosms in the experimental evolution. RNA was extracted from the soil samples and amplified using qPCR primers specific to the isolates which had been used to inoculate the microcosms.
- File identifier
- 4b27e5d5-2ec4-4b72-9939-ab66c2818612 XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Hierarchy level name
- nonGeographicDataset
- Date stamp
- 2025-03-21T09:39:08
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
