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Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms

This dataset contains information on the performance of soil eubacteria inoculated into soil microcosms and exposed to a series of experimental droughts (or control conditions) in an experimental evolution study. Isolates of Bacillus and Pseudomonas included in the experimental evolution study had been sampled from the roots of Festuca ovina in the control treatment at the Buxton Climate Change Impacts Lab (BCCIL). Variables measured included estimates of microbial population size (via qPCR), microcosm soil respiration in response to substrate addition (in a substrate utilisation profiling assay) and water loss from the soil in response to the experimental drought/control treatments. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612

Simple

Date (Publication)
2024-05-13
Date (Creation)
2023-03-10
Identifier
https://catalogue.ceh.ac.uk/id/4b27e5d5-2ec4-4b72-9939-ab66c2818612
Identifier
doi: / 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
Other citation details
Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre 10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
Author
  Jazz Pharmaceuticals - Hahn, C.Z.
https://orcid.org/0000-0002-8399-4682
Author
  University of Liverpool - Airey, G.A.
Author
  Woodland Trust - Crawford, J.A.
Author
  Lancaster University - Sayer, E.J.
https://orcid.org/0000-0002-3322-4487
Author
  Lancaster University - Stevens, C.J.
https://orcid.org/0000-0002-2390-1763
Author
  University of Liverpool - Paterson, S.
https://orcid.org/0000-0002-1307-2981
Author
  University of Liverpool - Whitlock, R.
https://orcid.org/0000-0002-7067-8365
Point of contact
  University of Liverpool - Whitlock, R.
https://orcid.org/0000-0002-7067-8365
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC EDS Environmental Information Data Centre
Owner
  University of Liverpool
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Hahn, C.Z., Airey, G.A., Crawford, J.A., Sayer, E.J., Stevens, C.J., Paterson, S., Whitlock, R. (2024). Growth and physiological responses of grassland soil bacteria (Bacillus and Pseudomonas) to drought treatment in experimental microcosms. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Biota
  • Environment
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
Bacterial isolates from BCCIL (twelve from each of genera Bacillus and Pseudomonas) were raised in liquid media and used to inoculate sterilised soil in kilner jars (approx 100 ml soil in 500 ml jars). Each isolate of each bacterial taxon was inoculated into five replicate microcosms per moisture treatment (drought and control) and microcosms were assigned random positions within four experimental blocks. Microcosms in the control treatment were topped up weekly to a volumetric water content of 40%. Those in the drought treatment were subjected to a series of four simulated droughts lasting six weeks, where watering was not carried out. Soil moisture content determination and watering was carried out by assessing the mass of each kilner jar and the dried soil it initially contained, allowing controlled watering to the target soil water content. Substrate utilisation profiling was carried out by taking soil samples from the microcosms at the end of the final drought cycle, which were amended with different substrates (water, glucose, cellulose, artificial root exudate)in MicroResp(TM) colourimetric microplates, before CO2 efflux detection on a FLUOstar Omega spectrophotometer (BMG Labtech, Ortenberg, Germany). q-PCR was carried out by taking soil samples from the microcosms in the experimental evolution. RNA was extracted from the soil samples and amplified using qPCR primers specific to the isolates which had been used to inoculate the microcosms.
File identifier
4b27e5d5-2ec4-4b72-9939-ab66c2818612 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-03-21T09:39:08
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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