Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a high glucose concentration substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured. All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology. The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability. The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf. Full details about this dataset can be found at https://doi.org/10.5285/a15c35d7-0247-4ea3-820b-f6f20cc5ffed

General soil chemical, physical and microbial properties are presented for soil samples at six depths collected from a field experiment in the Conwy catchment. Samples were collected in May 2015. Core samples were taken along three transects by trained members of staff the Centre for Ecology & Hydrology and Bangor University. General soil chemical, physical and microbial properties were measured at six depths by trained members of staff, using recognised procedures at the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. The samples were taken to provide supporting information for assessing the relationship between soil microbial populations and soil properties. The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf. Full details about this dataset can be found at https://doi.org/10.5285/f7b2beaa-08cf-48db-9b07-c55118d11506

Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a low glucose concentration substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured. All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology. The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability. The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf. Full details about this dataset can be found at https://doi.org/10.5285/c29d2ce1-0295-439b-adf1-98ed59f73fae