soil ecology
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This dataset contains soil microbial carbon cycling parameters (growth, respiration, carbon use efficiency) using O18-incorporation into DNA method, microbial biomass by fumigation-extraction), bacterial and fungal necromass concentrations (by amino sugars), soil chemical properties (nutrients, pH), and soil extracellular enzyme activities and climate properties for two experimental soil systems. All measurements are made across two experimental systems over a 3.4 km elevation gradient in the Peruvian Andes (Manu National Park). The first system includes measurements for 14 x 1 ha permanent sampling plots situated across the 3.4 km elevation gradient. The second system includes measurements for a soil translocation experiment where soil cores were translocated reciprocally across 4 sites spanning the gradient; representing a temperature manipulation experiment. The measurements were collected after 11 years of temperature manipulation, in 2020-2021. This dataset was created to understand how temperature affects microbial carbon cycling process in soils, across two different time-frames (long-term vs 11-years of climate perturbation). Full details about this dataset can be found at https://doi.org/10.5285/4077c5f8-9a36-4112-8a86-604ccffbb363
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This dataset contains information on the performance of soil eubacteria inoculated into soil microcosms and exposed to a series of experimental droughts (or control conditions) in an experimental evolution study. Isolates of Bacillus and Pseudomonas included in the experimental evolution study had been sampled from the roots of Festuca ovina in the control treatment at the Buxton Climate Change Impacts Lab (BCCIL). Variables measured included estimates of microbial population size (via qPCR), microcosm soil respiration in response to substrate addition (in a substrate utilisation profiling assay) and water loss from the soil in response to the experimental drought/control treatments. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4b27e5d5-2ec4-4b72-9939-ab66c2818612
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This dataset contains information on the performance of soil microbial communities (from field soil washes), soil synthetic microbial populations (from isolates within a single bacterial genus) and plants each originating from either drought and control long-term climate treatments at the Buxton Climate Change Impacts Lab (BCCIL), in a microcosm experiment. Plant and microbe provenance were manipulated to effect different combinations of climate ancestry in different microcosm pots. In addition, a factorial watering treatment (control or drought treatment) was applied. Isolates of Pseudomonas used to found experimental populations of soil microbes had been sampled from the roots of Festuca ovina in the control and drought treatments at BCCIL. Variables measured included soil respiration, plant traits, plant available soil nutrients, soil enzyme activities, soil respiration responses to substrate addition (in a substrate utilisation profiling assay), and soil respiration responses to drought (in a substrate utilisation profiling assay). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/e09db3ad-c54a-4f99-bf3c-37ee63fc2998
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