This resource holds survival data of human pathogens bound to microplastics during transfer through the freshwater-marine continuum. The survival of Escherichia coli, Enterococcus faecalis and Pseudomonas aeruginosa colonising polyethylene or glass particles was quantified in mesocosm incubation experiments designed to simulate, (1) the direct release of microplastics from wastewater treatment plants (WWTPs) into freshwater and seawater environments; and (2) the movement of microplastics downstream following discharge from the WWTP through the river-estuary-marine-beach continuum. Background bacterial concentrations and crystal violet were also measured. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/c31c0b2a-ee5b-479f-84c3-ff1b0bfc6d84

The resource contains experimental data collected at the Institute of Ecology and Evolution, University of Edinburgh between Jan-Oct 2023. Sixty (60) lines from the Drosophila Genetic Reference Panel (DGRP) were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed. Total number of activity events were measured for each fly individually using Drosophila Activity Monitor (DAM), measured at 1 min resolution until death. Mortality was recorded as the last detected movement in DAM. Pathogen shedding was measured for each individual fly as the number of viable P. aeruginosa colony-forming units defecated by an infected fly for 24h following oral infection. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b