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Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa

The resource contains experimental data collected at the Institute of Ecology and Evolution, University of Edinburgh between Jan-Oct 2023. Sixty (60) lines from the Drosophila Genetic Reference Panel (DGRP) were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed. Total number of activity events were measured for each fly individually using Drosophila Activity Monitor (DAM), measured at 1 min resolution until death. Mortality was recorded as the last detected movement in DAM. Pathogen shedding was measured for each individual fly as the number of viable P. aeruginosa colony-forming units defecated by an infected fly for 24h following oral infection. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b

Simple

Date (Publication)
2024-05-08
Date (Creation)
2024-04-10
Identifier
https://catalogue.ceh.ac.uk/id/65c7085a-7bcf-481a-884a-8e1c9cfc395b
Identifier
doi: / 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b
Other citation details
Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre 10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b
Author
  University of Edinburgh - Vale, P.
https://orcid.org/0000-0003-4558-9202
Point of contact
  University of Edinburgh - Vale, P.
https://orcid.org/0000-0003-4558-9202
Author
  University of Edinburgh - Kutzer, M.
https://orcid.org/0000-0002-8696-6978
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC EDS Environmental Information Data Centre
Owner
  University of Edinburgh
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Vale, P., Kutzer, M. (2024). Drosophila activity, survival and pathogen shedding after oral infection with the bacterial pathogen Pseudomonas aeruginosa. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/65c7085a-7bcf-481a-884a-8e1c9cfc395b
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Biota
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
Sixty (60) lines from the DGRP were exposed to an orally acquired infection of the bacterial pathogen Pseudomonas aeruginosa. Six to ten replicate flies for each line (60) and sex (2) were exposed individually to infection overnight. Each treatment was replicated 5-10 times (707 samples in total). The experiment was split among 5 experimental blocks with a variable number of replicates tested in each, as described in the data file. Following infection (see above) pathogen shedding was measured by placing each individual fly inside a sterile Eppendorf and then quantifying the number of viable P. aeruginosa colony-forming units defecated each infected fly for 24h following oral infection. CFU (colony forming unit) plating was carried out on Pseudomonas sp. selective media (PIM) to select for P. aeruginosa CFUs only. Each fly was then transferred to Drosophila Activity Monitor (DAM) and the total number of activity events were measured for each fly individually measured at 1 min resolution until death (2500-~20,000 minutes). Data generated in the DAM included the total number of activity bouts recoded for each minute for the duration of the experiment (2500-~20,000 minutes). The values reported in the resource are the total number of activity bouts recoded per fly until death or until the end of the experiment. Mortality was recorded as the last detected movement in DAM.
File identifier
65c7085a-7bcf-481a-884a-8e1c9cfc395b XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-11-13T16:23:23
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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