This dataset contains locations of worker bumblebees of five species (Bombus terrestris, B. lapidarius, B. pascuorum, B. hortorum, B. ruderatus) across an agricultural landscape centred on the Hillesden Estate, Buckinghamshire, UK. Locations were recorded in the field using a handheld GPS unit. Workers were non-lethally DNA sampled between June and August 2011, and genetic analysis used to confirm species and assign individuals to full-sib groups (colonies). Data were collected as part of a project led by the Centre for Ecology & Hydrology, funded under the Insect Pollinators Initiative. Full details about this dataset can be found at https://doi.org/10.5285/a60f52b8-0f9f-44f6-aca4-861cb461a0eb

Land use/land cover (LULC) map of a 20 km^2 agricultural landscape centred on the Hillesden Estate, Buckinghamshire, UK. The map is based on remote sensed data (LiDAR and hyperspectral sensors) with manual updates and the addition of spring and summer floral cover data from comprehensive field surveys. The remote sensed data was generated in August 2007. The manual updates and summer floral data were from field visits in July and August 2011, and the spring floral data were from field visits in April 2011 and 2012. The map was created as part of a project led by the Centre for Ecology & Hydrology, funded under the Insect Pollinators Initiative. Full details about this dataset can be found at https://doi.org/10.5285/0667cf06-f2c3-45c1-a80a-e48539b52427

Microsatellite data for five species of common and declining bumblebee (Bombus terrestris, B. lapidarius, B. pascuorum, B. hortorum and B. ruderatus) collected across the Hillesden Estate, Buckinghamshire, UK, in summer 2011. Worker genotypes were determined from individuals sampled across an agricultural landscape and queen genotypes were reconstructed from sampled worker offspring. Data were collected as part of a project led by the Centre for Ecology & Hydrology, funded under the Insect Pollinators Initiative. Full details about this dataset can be found at https://doi.org/10.5285/6a408415-0575-49c6-af69-b568e343266d