This dataset contains photographs of pairs of Streptomyces bacteria and it was collected with the aim of interrogating the manner and means of the interactions. The four imaged Streptomyces were originally isolated from a nature reserve in Minnesota, USA. Images show the interaction phenotypes between each pairing (including self to self), with images taken daily on International Streptomyces Project-2 (ISP-2) agar. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/3f48eb4c-bc0d-49e3-af71-dcff6d88c534

This dataset contains features detected by untargeted metabolomics analysis of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains the mass-to-charge (m/z) and retention time of identified peaks, and their ion intensity in each sample. The relative abundance of these identified features was later compared between pairs of interacting strains to gain insight into changes in their metabolism. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4921f306-5efa-4bf7-a15e-a157c2665889