Microbial genomic DNA data for participant collected household vacuum dust from homes across two bioclimatic regions (UK and Greece) (NERC Grant NE/T004401/1)

The <250um fraction of 19 household vacuum dust samples (collected by citizen participants during 2019-2021) were extracted using high throughput isolation of microbial genomic DNA and sequenced using Illumina NextSeq (12 samples from a national campaign within the UK, 7 samples from Greece and a negative reagent control included to ensure sterility throughout the processing and sequencing steps). These data are available (following period of embargo) from the European Nucleotide Archive via the individual sample accession numbers ERS9609044 to ERS9609063, submitted under the study ID PRJEB49546. Sample location data are provided at town/city, country level. Given the amount of time people spend indoors, residential environments are perhaps the most important, but understudied environments with respect to human exposure to microbes and other contaminants. Across our urban environments, anthropogenic activities (both current and legacy) provide for multiple sources and pathways for the generation and distribution of microbes, inorganic and organic contaminants within the home environment, yet we know relatively little about the potential for dissemination of antibiotic resistance in microbial communities within indoor dust.

Date (Creation): 2022-01-04

Identifier: http://data.bgs.ac.uk/id/dataHolding/13607876

Originator

Northumbria University - Jane Entwistle

Department of Geography and Environmental Sciences, Ellison Building , Newcastle upon Tyne , NE1 8ST ,

Principal investigator

Northumbria University - Jane Entwistle

Department of Geography and Environmental Sciences, Ellison Building , Newcastle upon Tyne , NE1 8ST ,

Distributor

British Geological Survey - Enquiries

Point of contact

British Geological Survey - Enquiries

Maintenance and update frequency: notApplicable notApplicable

GEMET - INSPIRE themes, version 1.0

Geology

BGS Thesaurus of Geosciences

Dust
NGDC Deposited Data

dataCentre

NGDC Deposited Data

Keywords

NERC_DDC

Access constraints: otherRestrictions Other restrictions

Other constraints: restricted

Other constraints: The dataset has been formally approved by BGS for access by external clients under a BGS Digital Data Licence, issued via BGS's IPR Section. The dataset must not be provided to, or be accessible by, an external client until the IPR Section confirms that signed licence documentation is in place. Refer to the IPR Section (DigitalLE@bgs.ac.) if further advice is required.. Constraint applies until 2022-12-31

Use constraints: otherRestrictions Other restrictions

Other constraints: The copyright of materials derived from the British Geological Survey's work is vested in the Natural Environment Research Council [NERC]. No part of this work may be reproduced or transmitted in any form or by any means, or stored in a retrieval system of any nature, without the prior permission of the copyright holder, via the BGS Intellectual Property Rights Manager. Use by customers of information provided by the BGS, is at the customer's own risk. In view of the disparate sources of information at BGS's disposal, including such material donated to BGS, that BGS accepts in good faith as being accurate, the Natural Environment Research Council (NERC) gives no warranty, expressed or implied, as to the quality or accuracy of the information supplied, or to the information's suitability for any use. NERC/BGS accepts no liability whatever in respect of loss, damage, injury or other occurence however caused.

Other constraints: Either: (i) the dataset is made freely available, e.g. via the Internet, for a restricted category of use (e.g. educational use only); or (ii) the dataset has not been formally approved by BGS for access and use by external clients under licence, but its use may be permitted under alternative formal arrangements; or (iii) the dataset contains 3rd party data or information obtained by BGS under terms and conditions that must be consulted in order to determine the permitted usage of the dataset. Refer to the BGS staff member responsible for the creation of the dataset if further advice is required. He / she should be familiar with the composition of the dataset, particularly with regard to 3rd party IPR contained in it, and any resultant use restrictions. This staff member should revert to the IPR Section (ipr@bgs.ac.uk ) for advice, should the position not be clear.

Metadata language: EnglishEnglish

Topic category

Geoscientific information

Begin date: 2020-10-05

End date: 2020-10-30

Reference System Information

No information provided.

Distribution format

MS Excel ()

Distributor

British Geological Survey - Enquiries

OnLine resource: Data

Hierarchy level: nonGeographicDataset Non geographic dataset

Other: non geographic dataset

Conformance result

Date (Publication): 2011

Explanation: See the referenced specification

Pass: No

Conformance result

Date (Publication): 2010-12-08

Explanation: See http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:323:0011:0102:EN:PDF

Pass: No

Statement: High throughput isolation of microbial genomic DNA from each <250 um citizen-collected vacuum dust sample was performed using a DNeasy 96 PowerSoil Pro Kit (384) (QIAGEN). Metagenomic library preparation was performed using Nextera XT DNA Library Preparation Kit. Sequencing was performed in-house (Northumbria University) using an Illumina NextSeq with a Mid-Output 300 cycle kit (150 base paired end reads), to facilitate a sequencing depth of 10M reads per sample. A negative reagent controls were included to ensure sterility throughout the processing and sequencing steps. Adaptor sequence trimming and quality filtering was performed with TrimGalore. Reads shorter than 100bp were removed in addition to sequences aligning to the human genome (removed using BBMap). After sequence filtering and quality control, a median/mean 4419820/4784250 sequence reads per sample remained.