E. coli counts of fresh cattle faeces from the North Wyke Farm Platform
The data consists of faecal microbiology and moisture content taken from two separate cohorts of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected between November 2016 and July 2018. Samples were collected and microbiologically analysed in the laboratory within 6 to 8 hours. Two cohorts of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Each cohort covering the period that cattle enter the farm platform, i.e. from weaning until slaughter ca. 16 – 20 months. Full details about this dataset can be found at
https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
INSPIRE
Identification
- File identifier
- ec83a4ea-923a-4f02-8a9f-aeecc43d7123 XML
- Resource type
- dataset
Online resource
Resource identifier
- Metadata Language
- English (en)
- Spatial representation type
- textTable
Encoding
- Format
- Comma-separated values (CSV)
Projection
- code
- WGS 84
Classification of data and services
- Topic category
-
- Biota
- Farming
- Environment
Classification of data and services
Coupled resource
Coupled resource
Classification of data and services
Coupled resource
Coupled resource
Keywords
- GEMET - INSPIRE themes, version 1.0 ()
-
- Environmental Monitoring Facilities
Geographic coverage
N
S
E
W
Temporal reference
Temporal extent
Temporal extent
- Dataset Reference Date ()
- 2019-09-13
Quality and validity
- Lineage
- Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory one gram of faecal material was aseptically transferred to nine ml of sterile Ringers (Oxoid, Basingstoke, UK) and appropriate serial 10-fold dilutions were made. An aliquot (0.1 ml) was plated in duplicate on to Membrane Lactose Glucuronide Agar (MLGA) (Oxoid) and spread across the surface of the agar using a disposal plastic spreader (VWR). Antibiotics were added to the MLGA at suitable concentrations after autoclaving and prior to pouring the plates. Antibiotics were added at the following rates; 16 mg/l for tetracycline and cephalexin, 1.0 mg/l marbofloxacin, 8 mg/l meropenem. All plates were allowed to air dry prior to inversion and incubation, at 44.5 ◦C (±0.2 ◦C) for 18–24 h. Colonies were counted, after incubation, and reported as E.coli colony forming units (CFU) per gram fresh weight and also on a dry weight basis. All colony counts were further transformed by log to base 10. Gravimetric moisture content of faecal samples, approximately 50 g of faecal material was accurately weighed into a pre-weighed silver foil container and dried in an oven at 105 ◦C, until constant weight. To calculate % dry matter: 100 – (((fresh wt.-Dry wt.) /(fresh wt.))*100), effectively 100 – moisture content. The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677. Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.
- Distance
- 5 urn:ogc:def:uom:EPSG::9001
Conformity
Conformity
Conformity
Conformity
Conformity
Conformity
Restrictions on access and use
- Access constraints
- no limitations
- Access constraints
- © Natural Environment Research Council
- Access constraints
- This resource is available under the terms of the Open Government Licence
- Access constraints
- If you reuse this data, you should cite: Hodgson, C. J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
Responsible organization (s)
Contact for the resource
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- NERC EDS Environmental Information Data Centre
- enquiries@ceh.ac.uk
- Organisation name
- NERC Environmental Information Data Centre
- enquiries@ceh.ac.uk
Responsible organization (s)
Contact for the resource
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- NERC EDS Environmental Information Data Centre
- enquiries@ceh.ac.uk
- Organisation name
- NERC Environmental Information Data Centre
- enquiries@ceh.ac.uk
Metadata information
Contact for the metadata
- Organisation name
- NERC EDS Environmental Information Data Centre
- info@eidc.ac.uk
- Metadata Date
- 2024-02-08T17:37:26
- Metadata Language
- English (en)
- Character set
- utf8