Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle
The data presented are quantitative polymerase chain reaction (qPCR) read outs from antimicrobial resistance gene (AMRG) assays and associated metadata from this project. In this dataset, the mean gene copy numbers per microlitre of DNA extract are shown. The data were collected from faecal and environmental samples which were obtained from a single British commercial pig unit. The former were collected from the sow housing barn, pig growing houses and slurry tanks within the farm unit and the latter were obtained through random stratified sampling of the farm and the surrounding land. These samples were taken from what will be referred to as the 'main study'. A further study was carried out to obtain samples after a partial depopulation which took place on this farm. Faecal samples were obtained from the sow housing barn, pig growing houses and slurry tanks and will be referred to as the 'depopulation (depop) study'. For the main study, the samples were collected between October 19th 2016 and April 5th 2017. For the depop study, the samples were collected between 19th June 2017 and 13th November 2017. The data associated with all samples were generated between August 1st 2017 and May 1st 2018. Full details about this dataset can be found at
https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
- Identification
- Distribution
- Quality
- Spatial rep.
- Ref. system
- Content
- Portrayal
- Metadata
- Md. constraints
- Md. maintenance
- Schema info
Identification
Data identification
Citation
- Dataset Reference Date ()
- 2018-08-10
- Identifier
- doi: / 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
- Other citation details
- Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
- GEMET - INSPIRE themes, version 1.0 ()
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- Soil
- Agricultural and Aquaculture Facilities
- Human Health and Safety
Legal constraints
- Limitations on Public Access
- otherRestrictions
- Other constraints
- no limitations
Legal constraints
- Use constraints
- otherRestrictions
Legal constraints
- Use constraints
- otherRestrictions
- Other constraints
- © University of Edinburgh
Legal constraints
- Use constraints
- otherRestrictions
- Other constraints
- If you reuse this data, you should cite: Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
- Spatial representation type
- textTable
- Metadata Language
- English (en)
- Character set
- utf8
- Topic category
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- Biota
- Farming
- Begin date
- 2016-10-19
- End date
- 2017-04-05
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E
W
Distribution
Distribution
- Data format
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Comma-separated values (CSV)
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Comma-separated values (CSV)
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Download the data
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Supporting information
Supporting information available to assist in re-use of this dataset
Quality
Data quality
Scope
- Quality Scope
- dataset
- Other
- dataset
Report
Citation
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Faecal and soil samples were collected from the study farm weekly using spooned universal tubes and were stored at -20 deg C, batch transported on dry ice to the laboratory where they were stored at -80 deg C until DNA extraction was carried out. DNA extracts were quantified and assessed using a NanoDrop spectrophotometer and split into aliquots for storage. DNA extracts were then screened in triplicate by qPCR and the number of gene copies assessed through the use of a standard curve generated by the inclusion of standards with known copy numbers of each target gene. Non-template controls were also included to ensure that non-specific binding or contamination were not occurring. The qPCR machine software (i.e. MxPro) was then used to calculate the number of gene copies in each of the collected samples and the mean was calculated using the three replicates. These values are included in the data set provided.
Ref. system
Metadata
Metadata
- File identifier
- e548dc5d-49e3-467d-9435-c199da40e7be XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-03-12T11:03:16
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/