Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017)
The data consist of nitrogen gene data, soil biodiversity indices and microbial community composition for three soil depths (0-15, 15-30 and 30-60 cm) from a winter wheat field experiment located in the United Kingdom and collected between April 2017 and August 2017. The sites were Rothamsted Research at North Wyke in Devon and Bangor University at Henfaes Research Station in North Wales. At each site measurements were taken from 15 plots, organised within a randomised complete block design where 5 plots did not receive fertilizers (controls), 5 plots received food-based digestate, and 5 plots received acidified food based digestate a nitrification inhibitor. Soil samples were taken within two weeks of digestate application and shortly before winter wheat harvest. Soil chemical parameters were: soil nitrate, ammonium, dissolved organic carbon and nitrogen, amino acids and peptides, soil organic matter content as loss-on-ignition, pH, sodium, potassium, calcium, magnesium, permanganate oxdisable carbon citric acid extractable phosphorous, Olsen-P and total carbon, nitrogen and phosphorus. Soil biological measure were: microbial biomass carbon and nitrogen. Soil samples were taken by members of staff from Centre of Ecology & Hydrology (Bangor), Bangor University, School of Environment, Natural Resources & Geography Sustainable Agricultural Sciences, and Rothamsted Research North Wyke. Measurements were carried out Rothamsted Research Harpenden and the Centre of Ecology & Hydrology (Wallingford). Soil physico-chemical parameters were measured on the same soil samples and are presented in a related dataset. https://catalogue.ceh.ac.uk/id/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab Data was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC - Ref BB/N013468/1 Full details about this dataset can be found at
https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca
INSPIRE
Identification
- File identifier
- 391c0294-07f1-4856-b592-428bd44055ca XML
- Resource type
- dataset
Online resource
Resource identifier
- Metadata Language
- English (en)
- Spatial representation type
- textTable
Encoding
- Format
- Comma-separated values (CSV)
Projection
- code
- WGS 84
Classification of data and services
- Topic category
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- Farming
Classification of data and services
Coupled resource
Coupled resource
Classification of data and services
Coupled resource
Coupled resource
Keywords
- GEMET - INSPIRE themes, version 1.0 ()
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- Environmental Monitoring Facilities
Geographic coverage
N
S
E
W
N
S
E
W
Temporal reference
Temporal extent
Temporal extent
- Dataset Reference Date ()
- 2019-04-23
Quality and validity
- Lineage
- Soil samples were taken by members of staff from the different sites. Samples were sent to Rothamsted Research Harpenden for DNA extractions and extracts were analysed for nitrogen genes (nifH, amoA, nirK, nirS, nosZ, ureC). Extracts were also sent to CEH Wallingford for analyses of operational taxonomic units (OTUs'). Soil community DNA was extracted from 0.25 grams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific). Quantitative-Polymerase chain reaction (qPCR) protocols were followed as detailed in De Sosa et al. 2018. Extracted DNA was supplied from Rothamsted Research in Harpenden for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to 16S Ribosomal ribonucleic acid (rRNA) amplicon sequencing as described in Kozich 2013 and Internal transcribed spacer (ITS) rRNA amplicon sequencing employing the Kozich 2013 strategy and Ihrmark 2012. All results were entered into a Microsoft Excel spreadsheet and converted to .csv files for ingestion into the Environmental Information Data Centre.
Conformity
Conformity
Conformity
Conformity
Conformity
Conformity
Restrictions on access and use
- Access constraints
- no limitations
- Access constraints
- © Rothamsted Research
- Access constraints
- © UK Centre for Ecology & Hydrology
- Access constraints
- © Bangor University
- Access constraints
- If you reuse this data, you should cite: Goodall, T.I., Griffiths, R.I., Blaud, A, Abadie, M., Clark, I.M., Hirsch, P.R., Carswell, A.M., Misselbrook, T.H., Sánchez-Rodríguez, A.R., Chadwick, D.R., Jones, D.L., Reinsch, S. (2019). Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca
Responsible organization (s)
Contact for the resource
- Organisation name
- UK Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Bangor University
- enquiries@ceh.ac.uk
- Organisation name
- Bangor University
- enquiries@ceh.ac.uk
- Organisation name
- Bangor University
- enquiries@ceh.ac.uk
- Organisation name
- Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- NERC EDS Environmental Information Data Centre
- enquiries@ceh.ac.uk
- Organisation name
- NERC Environmental Information Data Centre
- enquiries@ceh.ac.uk
Responsible organization (s)
Contact for the resource
- Organisation name
- UK Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Rothamsted Research
- enquiries@ceh.ac.uk
- Organisation name
- Bangor University
- enquiries@ceh.ac.uk
- Organisation name
- Bangor University
- enquiries@ceh.ac.uk
- Organisation name
- Bangor University
- enquiries@ceh.ac.uk
- Organisation name
- Centre for Ecology & Hydrology
- enquiries@ceh.ac.uk
- Organisation name
- NERC EDS Environmental Information Data Centre
- enquiries@ceh.ac.uk
- Organisation name
- NERC Environmental Information Data Centre
- enquiries@ceh.ac.uk
Metadata information
Contact for the metadata
- Organisation name
- NERC EDS Environmental Information Data Centre
- info@eidc.ac.uk
- Metadata Date
- 2024-02-27T16:17:31
- Metadata Language
- English (en)
- Character set
- utf8
SDS
Conformance class 1: invocable
Access Point URL
Endpoint URL
Technical specification
Conformance class 2: interoperable
Access constraints
Limitation
Use constraints
Limitation
Responsible custodian
Contact for the resource
Overviews
N
S
E
W
N
S
E
W
Publishing Body
Associated resources (if any)
Not available