391c0294-07f1-4856-b592-428bd44055ca
English
ISO/IEC 8859-1 (also known as Latin 1)
dataset
dataset
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
Lancaster
LA1 4AP
UK
info@eidc.ac.uk
https://eidc.ac.uk/
EIDC website
The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences.
information
pointOfContact
2024-02-27T16:17:31
UK GEMINI
2.3
WGS 84
Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017)
2019-04-23
publication
https://catalogue.ceh.ac.uk/id/391c0294-07f1-4856-b592-428bd44055ca
10.5285/391c0294-07f1-4856-b592-428bd44055ca
doi:
Goodall, T.I., Griffiths, R.I., Blaud, A, Abadie, M., Clark, I.M., Hirsch, P.R., Carswell, A.M., Misselbrook, T.H., Sánchez-Rodríguez, A.R., Chadwick, D.R., Jones, D.L., Reinsch, S. (2019). Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017). NERC Environmental Information Data Centre 10.5285/391c0294-07f1-4856-b592-428bd44055ca
The data consist of nitrogen gene data, soil biodiversity indices and microbial community composition for three soil depths (0-15, 15-30 and 30-60 cm) from a winter wheat field experiment located in the United Kingdom and collected between April 2017 and August 2017. The sites were Rothamsted Research at North Wyke in Devon and Bangor University at Henfaes Research Station in North Wales. At each site measurements were taken from 15 plots, organised within a randomised complete block design where 5 plots did not receive fertilizers (controls), 5 plots received food-based digestate, and 5 plots received acidified food based digestate a nitrification inhibitor. Soil samples were taken within two weeks of digestate application and shortly before winter wheat harvest. Soil chemical parameters were: soil nitrate, ammonium, dissolved organic carbon and nitrogen, amino acids and peptides, soil organic matter content as loss-on-ignition, pH, sodium, potassium, calcium, magnesium, permanganate oxdisable carbon citric acid extractable phosphorous, Olsen-P and total carbon, nitrogen and phosphorus. Soil biological measure were: microbial biomass carbon and nitrogen. Soil samples were taken by members of staff from Centre of Ecology & Hydrology (Bangor), Bangor University, School of Environment, Natural Resources & Geography Sustainable Agricultural Sciences, and Rothamsted Research North Wyke. Measurements were carried out Rothamsted Research Harpenden and the Centre of Ecology & Hydrology (Wallingford). Soil physico-chemical parameters were measured on the same soil samples and are presented in a related dataset. https://catalogue.ceh.ac.uk/id/90df9dfa-a0c8-4ead-a13d-0a0a13cda7ab Data was collected for the Newton Fund project “UK-China Virtual Joint Centre for Improved Nitrogen Agronomy”. Funded by Biotechnology and Biological Sciences Research Council (BBSRC) and NERC - Ref BB/N013468/1 Full details about this dataset can be found at https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca
Reinsch, S.
UK Centre for Ecology & Hydrology
enquiries@ceh.ac.uk
https://orcid.org/0000-0003-4649-0677
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
pointOfContact
Goodall, T.I.
Centre for Ecology & Hydrology
enquiries@ceh.ac.uk
author
Griffiths, R.I.
Centre for Ecology & Hydrology
enquiries@ceh.ac.uk
author
Blaud, A
Rothamsted Research
enquiries@ceh.ac.uk
https://orcid.org/0000-0003-0860-8080
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
author
Abadie, M.
Rothamsted Research
enquiries@ceh.ac.uk
author
Clark, I.M.
Rothamsted Research
enquiries@ceh.ac.uk
https://orcid.org/0000-0002-1589-5420
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
author
Hirsch, P.R.
Rothamsted Research
enquiries@ceh.ac.uk
author
Carswell, A.M.
Rothamsted Research
enquiries@ceh.ac.uk
https://orcid.org/0000-0003-0580-6698
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
author
Misselbrook, T.H.
Rothamsted Research
enquiries@ceh.ac.uk
https://orcid.org/0000-0002-4594-3606
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
author
Sánchez-Rodríguez, A.R.
Bangor University
enquiries@ceh.ac.uk
https://orcid.org/0000-0001-8734-2035
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
author
Chadwick, D.R.
Bangor University
enquiries@ceh.ac.uk
author
Jones, D.L.
Bangor University
enquiries@ceh.ac.uk
https://orcid.org/0000-0002-1482-4209
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
author
Reinsch, S.
Centre for Ecology & Hydrology
enquiries@ceh.ac.uk
https://orcid.org/0000-0003-4649-0677
ORCID record
ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers.
information
author
NERC EDS Environmental Information Data Centre
enquiries@ceh.ac.uk
custodian
NERC Environmental Information Data Centre
enquiries@ceh.ac.uk
publisher
notPlanned
Environmental Monitoring Facilities
theme
GEMET - INSPIRE themes, version 1.0
2008-06-01
publication
otherRestrictions
no limitations
otherRestrictions
This resource is made available under the terms of the Open Government Licence
otherRestrictions
© Rothamsted Research
otherRestrictions
© UK Centre for Ecology & Hydrology
otherRestrictions
© Bangor University
otherRestrictions
If you reuse this data, you should cite: Goodall, T.I., Griffiths, R.I., Blaud, A, Abadie, M., Clark, I.M., Hirsch, P.R., Carswell, A.M., Misselbrook, T.H., Sánchez-Rodríguez, A.R., Chadwick, D.R., Jones, D.L., Reinsch, S. (2019). Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca
textTable
English
utf8
farming
2017-04-01
2017-10-31
-4.022
-4.011
53.236
53.241
-3.904
-3.892
50.767
50.775
Comma-separated values (CSV)
NERC EDS Environmental Information Data Centre
info@eidc.ac.uk
distributor
https://data-package.ceh.ac.uk/data/391c0294-07f1-4856-b592-428bd44055ca
Download the data
Download a copy of this data
download
https://data-package.ceh.ac.uk/sd/391c0294-07f1-4856-b592-428bd44055ca.zip
Supporting information
Supporting information available to assist in re-use of this dataset
information
dataset
dataset
Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services
2010-12-08
Soil samples were taken by members of staff from the different sites. Samples were sent to Rothamsted Research Harpenden for DNA extractions and extracts were analysed for nitrogen genes (nifH, amoA, nirK, nirS, nosZ, ureC). Extracts were also sent to CEH Wallingford for analyses of operational taxonomic units (OTUs'). Soil community DNA was extracted from 0.25 grams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific). Quantitative-Polymerase chain reaction (qPCR) protocols were followed as detailed in De Sosa et al. 2018. Extracted DNA was supplied from Rothamsted Research in Harpenden for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to 16S Ribosomal ribonucleic acid (rRNA) amplicon sequencing as described in Kozich 2013 and Internal transcribed spacer (ITS) rRNA amplicon sequencing employing the Kozich 2013 strategy and Ihrmark 2012. All results were entered into a Microsoft Excel spreadsheet and converted to .csv files for ingestion into the Environmental Information Data Centre.