Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017)
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Identification info
- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2019-04-23
- Identifier
- doi: / 10.5285/391c0294-07f1-4856-b592-428bd44055ca
- Other citation details
- Goodall, T.I., Griffiths, R.I., Blaud, A, Abadie, M., Clark, I.M., Hirsch, P.R., Carswell, A.M., Misselbrook, T.H., Sánchez-Rodríguez, A.R., Chadwick, D.R., Jones, D.L., Reinsch, S. (2019). Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017). NERC Environmental Information Data Centre 10.5285/391c0294-07f1-4856-b592-428bd44055ca
- Maintenance and update frequency
- notPlanned
- GEMET - INSPIRE themes, version 1.0 ()
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- Environmental Monitoring Facilities
- Limitations on Public Access
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- Other constraints
- no limitations
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- © Rothamsted Research
- Use constraints
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- © UK Centre for Ecology & Hydrology
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- © Bangor University
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- If you reuse this data, you should cite: Goodall, T.I., Griffiths, R.I., Blaud, A, Abadie, M., Clark, I.M., Hirsch, P.R., Carswell, A.M., Misselbrook, T.H., Sánchez-Rodríguez, A.R., Chadwick, D.R., Jones, D.L., Reinsch, S. (2019). Soil biological properties from a digestate experiment on winter wheat at North Wyke and Henfaes Farm, UK (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/391c0294-07f1-4856-b592-428bd44055ca
- Spatial representation type
- textTable
- Topic category
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- Farming
- Begin date
- 2017-04-01
- End date
- 2017-10-31
- Code
- WGS 84
Distribution Information
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- Quality Scope
- dataset
- Other
- dataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Soil samples were taken by members of staff from the different sites. Samples were sent to Rothamsted Research Harpenden for DNA extractions and extracts were analysed for nitrogen genes (nifH, amoA, nirK, nirS, nosZ, ureC). Extracts were also sent to CEH Wallingford for analyses of operational taxonomic units (OTUs'). Soil community DNA was extracted from 0.25 grams soil using the MoBio DNA PowerSoil DNA isolation kit (Mo Bio Laboratories, Inc. Carlsbad, CA), following manufacturer’s protocol. Extracted DNA was quantified by fluorometer Qubit® 2.0 dsDNA BR Assay Kit (Thermo Fisher Scientific) and quality checked by nanodrop (Thermo Fisher Scientific). Quantitative-Polymerase chain reaction (qPCR) protocols were followed as detailed in De Sosa et al. 2018. Extracted DNA was supplied from Rothamsted Research in Harpenden for amplicon sequencing at the facilities of the Molecular Ecology Group, Centre for Ecology & Hydrology, Wallingford. <50 μL volumes of DNA extract were supplied in 96-well plate format, these were subject to 16S Ribosomal ribonucleic acid (rRNA) amplicon sequencing as described in Kozich 2013 and Internal transcribed spacer (ITS) rRNA amplicon sequencing employing the Kozich 2013 strategy and Ihrmark 2012. All results were entered into a Microsoft Excel spreadsheet and converted to .csv files for ingestion into the Environmental Information Data Centre.
Metadata
- File identifier
- 391c0294-07f1-4856-b592-428bd44055ca XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-02-27T16:17:31
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3