Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016)
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Identification info
- Metadata Language
- English (en)
- Character set
- utf8
- Dataset Reference Date ()
- 2017-04-21
- Dataset Reference Date ()
- 2016-04-19
- Identifier
- doi: / 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
- Other citation details
- Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre 10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
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- no limitations
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- Use constraints
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- © The University of Edinburgh
- Use constraints
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- © Central Veterinary Research and Diagnostic Laboratory, Zimbabwe
- Use constraints
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- © Department of Livestock and Veterinary Services, Zimbabwe
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- If you reuse this data, you should cite: Goodwin, M., Webber, F., Hamill, L.C., Anderson, N.E., Shereni, W., Nyakupinda, L., Gwenhure, L.F., Macleod, E. (2017). Tsetse trypanosome and endosymbiont data from Hurungwe District, Zimbabwe (2016). NERC Environmental Information Data Centre https://doi.org/10.5285/1e435dd3-ed93-4961-a136-ffa477731e1c
- Spatial representation type
- textTable
- Topic category
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- Biota
- Health
- Begin date
- 2014-02-01
- End date
- 2016-04-12
- Code
- WGS 84
Distribution Information
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Comma-separated values (CSV)
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- Quality Scope
- dataset
- Other
- dataset
Report
- Dataset Reference Date ()
- 2010-12-08
- Statement
- The data presents the molecular analysis of 101 Glossina morsitans morsitans and 108 Glossina pallidipes tsetse sampled in Hurungwe District, Mashonaland West Province, Zimbabwe. Tsetse were sampled using Epsilon traps and fly rounds as described by Shereni et al. (2016). Tsetse were stored in cryotubes with desiccant beads at room temperature. DNA was extracted from the tsetse using Qiagen DNeasy kits and then subjected to polymerase chain reactions (PCR) to detect the primary endosymbiont, Wigglesworthia glossindia. Samples from which no DNA was amplified were discounted from further molecular analysis. All remaining samples were subjected to PCR reactions to detect African trypanosomes and the secondary tsetse symbionts, Sodalis glossinidius and Wolbachia. All PCRs included a negative and a positive control. The data were collated in an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC.
Metadata
- File identifier
- 1e435dd3-ed93-4961-a136-ffa477731e1c XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-03-01T11:26:07
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3