Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments
This datset contains operational taxonomic units for epilithon eukaryotes (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample. Full details about this dataset can be found at https://doi.org/10.5285/18023db5-25d8-44f7-b291-61869f937367
- Identification
- Distribution
- Quality
- Spatial rep.
- Ref. system
- Content
- Portrayal
- Metadata
- Md. constraints
- Md. maintenance
- Schema info
Identification
Data identification
Citation
- Dataset Reference Date ()
- 2014-05-06
- Dataset Reference Date ()
- 2010-01-01
- Identifier
- CEH:EIDC: / 1395928342078
- Identifier
- doi: / 10.5285/18023db5-25d8-44f7-b291-61869f937367
- Other citation details
- Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre 10.5285/18023db5-25d8-44f7-b291-61869f937367
Maintenance information
- Maintenance and update frequency
- unknown
- Maintenance note
- not planned
- GEMET - INSPIRE themes, version 1.0 ()
-
- Land Use
Legal constraints
- Limitations on Public Access
- otherRestrictions
- Other constraints
- no limitations
Legal constraints
- Use constraints
- otherRestrictions
Legal constraints
- Use constraints
- otherRestrictions
- Other constraints
- © UK Centre for Ecology & Hydrology
Legal constraints
- Use constraints
- otherRestrictions
- Other constraints
- If you reuse this data, you should cite: Griffiths, R., Edwards, F., Scarlett, P., Webb, G., Sarlej, R., Thomson, B. (2014). Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments. NERC Environmental Information Data Centre https://doi.org/10.5285/18023db5-25d8-44f7-b291-61869f937367
- Spatial representation type
- textTable
- Metadata Language
- English (en)
- Character set
- utf8
- Topic category
-
- Biota
- Environment
- Inland waters
- Location
- Begin date
- 2013-10-01
- End date
- 2014-03-20
N
S
E
W
Distribution
Distribution
- Data format
-
-
Comma-separated values (CSV)
()
-
Comma-separated values (CSV)
()
- Resource Locator
-
Download the data
Get a copy of this data
- Resource Locator
-
Supporting Information
Supporting information available to assist in re-use of this dataset
Quality
Data quality
Scope
- Quality Scope
- dataset
- Other
- dataset
Report
Citation
- Dataset Reference Date ()
- 2010-12-08
- Statement
- Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.
Ref. system
Metadata
Metadata
- File identifier
- 18023db5-25d8-44f7-b291-61869f937367 XML
- Metadata Language
- English (en)
- Character set
- ISO/IEC 8859-1 (also known as Latin 1)
- Resource type
- dataset
- Hierarchy level name
- dataset
- Metadata Date
- 2024-02-27T16:19:12
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/