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Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022

This dataset holds realistic environmental concentrations of potential pathogens colonising microplastic beads (nurdles) collected from ten Scottish beaches. Observed measures include concentrations and the present genes of Klebsiella bacteria isolated from the nurdles, as well as the salinity, electrical conductivity, turbidity, and polymer composition of the nurdles. In addition, an infection experiment was conducted to measure the virulence of the Klebsiella bacteria. Galleria melonella larvae were infected with the isolated Klebsiella bacteria and the number of living larvae was recorded. Full details about this dataset can be found at https://doi.org/10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940

Simple

Date (Publication)
2024-08-09
Identifier
https://catalogue.ceh.ac.uk/id/fdd72810-a57f-444f-83cf-f2351ef4c940
Identifier
doi: / 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940
Other citation details
Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940
Author
  University of Stirling - Metcalf, R.
https://orcid.org/0000-0002-1463-427X
Author
  University of Stirling - Messer, L.
Author
  University of Stirling - White, H.L.
Author
  University of Stirling - Ormsby, M.
Author
  University of Stirling - Matallana-Surget, S.
Author
  University of Stirling - Quilliam, R.
https://orcid.org/0000-0001-7020-4410
Point of contact
  University of Stirling - Metcalf, R.
Owner
  University of Stirling
Publisher
  NERC EDS Environmental Information Data Centre
Custodian
  NERC EDS Environmental Information Data Centre
GEMET - INSPIRE themes, version 1.0
  • Environmental Monitoring Facilities
Access constraints
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no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940
Spatial representation type
textTable Text, table
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Environment
Begin date
2022-11-01
End date
2022-11-30
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Unique resource identifier
OSGB 1936 / British National Grid
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
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Conformance result

Date (Publication)
2010-12-08
Statement
Microplastic beads were collected from ten Scottish beaches (Erskine, Yellowcraig, Largs, Irvine, Ayr, Turnberry, Montrose, Broughty Ferry, Portobello and Eyemouth) and bacteria were removed for quantification of target bacteria. Bacteria were quantified using selective media and positive colony-forming units (CFU) were enumerated. Water temperature and salinity at each site were also measured with a thermometer and a salinity refractometer (RGBS). Fourier Transform Infrared Spectrometry (FTIR) was conducted on a Thermo Scientific Nicolet is50 FTIR to identify the polymer composition of the nurdles. The virulence of the isolated Klebsiella was studied for one of the sites, Brought Ferry. Galleria mellonella model of infection was used to determine the virulence of each of the selected Klebsiella strains following their isolation from the microplastic beads and sand. After injection, larvae were incubated at and inspected 24, 48 and 72 h post-injection; larvae were considered dead when they did not respond to touch stimuli.
File identifier
fdd72810-a57f-444f-83cf-f2351ef4c940 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
dataset Dataset
Hierarchy level name
dataset
Date stamp
2025-11-05T08:44:42
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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GEMET - INSPIRE themes, version 1.0
Environmental Monitoring Facilities

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