Date (Publication)

2024-08-09

Identifier

https://catalogue.ceh.ac.uk/id/fdd72810-a57f-444f-83cf-f2351ef4c940

Identifier

doi: / 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940

Other citation details

Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre 10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940

GEMET - INSPIRE themes, version 1.0

• Environmental Monitoring Facilities

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no limitations

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This resource is available under the terms of the Open Government Licence

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If you reuse this data, you should cite: Metcalf, R., Messer, L., White, H.L., Ormsby, M., Matallana-Surget, S., Quilliam, R. (2024). Nurdle characteristics with associated bacterial concentrations and virulence of Klebsiella isolates from Scottish beaches, 2022. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/fdd72810-a57f-444f-83cf-f2351ef4c940

Spatial representation type

textTable Text, table

Metadata language

EnglishEnglish

Character set

utf8 UTF8

Topic category

• Environment

Begin date

2022-11-01

End date

2022-11-30

Unique resource identifier

OSGB 1936 / British National Grid

Distribution format

• Comma-separated values (CSV) ()

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dataset Dataset

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dataset

Statement

Microplastic beads were collected from ten Scottish beaches (Erskine, Yellowcraig, Largs, Irvine, Ayr, Turnberry, Montrose, Broughty Ferry, Portobello and Eyemouth) and bacteria were removed for quantification of target bacteria. Bacteria were quantified using selective media and positive colony-forming units (CFU) were enumerated. Water temperature and salinity at each site were also measured with a thermometer and a salinity refractometer (RGBS). Fourier Transform Infrared Spectrometry (FTIR) was conducted on a Thermo Scientific Nicolet is50 FTIR to identify the polymer composition of the nurdles. The virulence of the isolated Klebsiella was studied for one of the sites, Brought Ferry. Galleria mellonella model of infection was used to determine the virulence of each of the selected Klebsiella strains following their isolation from the microplastic beads and sand. After injection, larvae were incubated at and inspected 24, 48 and 72 h post-injection; larvae were considered dead when they did not respond to touch stimuli.

File identifier

fdd72810-a57f-444f-83cf-f2351ef4c940 XML

Metadata language

EnglishEnglish

Character set

ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1

Hierarchy level

dataset Dataset

Hierarchy level name

dataset

Date stamp

2025-11-05T08:44:42

Metadata standard name

UK GEMINI

Metadata standard version

2.3