Mean daily flow and water chemistry data collected from the Tarland Burn, recorded between 2000-2010. Water chemistry determinands measured include: total dissolved phosphorus (TDP), soluble reactive phosphorus (SRP), total phosphorous (TP), particulate phosphorus (PP), nitrate (NO3-N), ammonium (NH4-N) and suspended sediments (SS); water chemistry measurements are in units of mg l-1 for all determinands. Sampling for water chemistry took place at a weekly frequency between 2000 and the end of 2003, with some daily sampling during rainfall events. Daily samples were then collected between February 2004 and June 2005. After June 2005, infrequent irregular sampling took place, with fewer determinands. Mean daily flow, in m3s-1, was also recorded. The Tarland Burn is a tributary of the River Dee (northeast Scotland). The samples were collected at Coull (WGS84 57.111, -2.810; OSGB 351050, 802540). Full details about this dataset can be found at https://doi.org/10.5285/f9a69fcb-321d-4f65-a8cf-cfe789224c8f