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Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions

This dataset contains information about the number of bacterial colonies recovered from plastics beads that were incubated in a 96-well plate for 24 hours at 37 degrees Celsius. Each well in the plate had 1 bead composed of polycaprolactone in it and 150 microlitres of Isopropyl β- d-1-thiogalactopyranoside (IPTG) induced bacterial cell culture. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to trigger the cells to form biofilms, the expression of these genes was induced using IPTG. An empty plasmid was used as the control. The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015

Simple

Date (Publication)
2024-10-28
Identifier
https://catalogue.ceh.ac.uk/id/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
Identifier
doi: / 10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
Other citation details
Howard, S., McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre 10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
Point of contact
  Brunel University London - McCarthy, R.R.
Author
  Brunel University London - Howard, S.
Author
  Brunel University London - McCarthy, R.R.
Owner
  Brunel University London
Publisher
  NERC EDS Environmental Information Data Centre
Custodian
  NERC EDS Environmental Information Data Centre
GEMET - INSPIRE themes, version 1.0
  • Human Health and Safety
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is licensed under a Creative Commons Attribution 4.0 International Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Howard, S., McCarthy, R.R. (2024). Colony formation data of plastic-degrading bacteria on polycaprolactone (PCL) beads, following modification to induce biofilm formation, under laboratory conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/eef3a20c-cb4c-415f-b4be-4d1c31fb6015
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Health
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
Controls included bacteria with empty vectors that did not contain the genes required to induce biofilm formation. Colonies were recovered from plastic beads following incubation for 24 hours at 37 degrees Celsius with bacteria engineered to have higher levels of biofilm formation. Beads were subsequently removed, washed 3 times in PBS and subsequently sonicated for 10 minutes to remove bacterial biofilm. Serial dilutions were made from the PBS suspension and spotted in triplicate on Luria Broth (LB) agar containing appropriate antibiotics. Plates were incubated for 16 hours at 37 degrees Celsius before colonies were counted.
File identifier
eef3a20c-cb4c-415f-b4be-4d1c31fb6015 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-06-04T07:07:29
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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GEMET - INSPIRE themes, version 1.0
Human Health and Safety

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