NERC Data Catalogue Service
E. coli counts of fresh cattle faeces from the North Wyke Farm Platform
The data consists of faecal microbiology and moisture content taken from two separate cohorts of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected between November 2016 and July 2018. Samples were collected and microbiologically analysed in the laboratory within 6 to 8 hours. Two cohorts of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Each cohort covering the period that cattle enter the farm platform, i.e. from weaning until slaughter ca. 16 – 20 months. Full details about this dataset can be found at
https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
Simple
- Date (Publication)
- 2019-09-13
- Identifier
- doi: / 10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
- Other citation details
- Hodgson, C.J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre 10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
- GEMET - INSPIRE themes, version 1.0
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- Environmental Monitoring Facilities
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- no limitations
- Use constraints
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- © Natural Environment Research Council
- Use constraints
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- If you reuse this data, you should cite: Hodgson, C.J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123
- Spatial representation type
- textTable Text, table
- Distance
- 5 urn:ogc:def:uom:EPSG::9001
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
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- Biota
- Farming
- Environment
- Begin date
- 2016-11-15
- End date
- 2018-07-17
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E
W
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- Unique resource identifier
- WGS 84
- Distribution format
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Comma-separated values (CSV)
()
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Comma-separated values (CSV)
()
- OnLine resource
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- Hierarchy level
- dataset Dataset
- Other
- dataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory one gram of faecal material was aseptically transferred to nine ml of sterile Ringers (Oxoid, Basingstoke, UK) and appropriate serial 10-fold dilutions were made. An aliquot (0.1 ml) was plated in duplicate on to Membrane Lactose Glucuronide Agar (MLGA) (Oxoid) and spread across the surface of the agar using a disposal plastic spreader (VWR). Antibiotics were added to the MLGA at suitable concentrations after autoclaving and prior to pouring the plates. Antibiotics were added at the following rates; 16 mg/l for tetracycline and cephalexin, 1.0 mg/l marbofloxacin, 8 mg/l meropenem. All plates were allowed to air dry prior to inversion and incubation, at 44.5 ◦C (±0.2 ◦C) for 18–24 h. Colonies were counted, after incubation, and reported as E.coli colony forming units (CFU) per gram fresh weight and also on a dry weight basis. All colony counts were further transformed by log to base 10. Gravimetric moisture content of faecal samples, approximately 50 g of faecal material was accurately weighed into a pre-weighed silver foil container and dried in an oven at 105 ◦C, until constant weight. To calculate % dry matter: 100 – (((fresh wt.-Dry wt.) /(fresh wt.))*100), effectively 100 – moisture content. The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677. Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.
- File identifier
- ec83a4ea-923a-4f02-8a9f-aeecc43d7123 XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- dataset Dataset
- Hierarchy level name
- dataset
- Date stamp
- 2025-11-13T16:15:32
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
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