This dataset contains hydrographic profiles (temperature, salinity, oxygen, fluorometer, transmissometer, irradiance) and along track measurements (bathymetry, surface meteorology, sea surface hydrography), with discrete measurements including water chemistry (organic and inorganic nutrients, particulate organic carbon and nitrogen, dissolved gases, trace metals) collected from a hydrographic section in the North Atlantic Ocean. This hydrographic section, designated A05 by the World Ocean Circulation Experiment (WOCE), runs along a nominal latitude of 24.5N between Florida and either Spain, North Africa, Portugal or the Canary Islands. Four UK cruises (D279, D346, DY040 and JC191) have contributed to this dataset to date using CTD casts, vessel-mounted and lowered ADCPs, bottle sampling and meteorological measuring systems to collect data. The measurements were collected as one of the UK's contributions to the Global Ocean Ship-based Hydrographic Investigations Program (GO-SHIP) and with the aim of contributing to the study of decadal variability of the present ocean circulation and meridional transport of heat, freshwater and biogeochemistry, as part of the Climate Linked Atlantic Sector Science (CLASS) project. The work was led by teams from the National Oceanography Centre at Southampton. Data from the section are held at the British Oceanographic Data Centre (BODC).