Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle

The data presented are quantitative polymerase chain reaction (qPCR) read outs from antimicrobial resistance gene (AMRG) assays and associated metadata from this project. In this dataset, the mean gene copy numbers per microlitre of DNA extract are shown. The data were collected from faecal and environmental samples which were obtained from a single British commercial pig unit. The former were collected from the sow housing barn, pig growing houses and slurry tanks within the farm unit and the latter were obtained through random stratified sampling of the farm and the surrounding land. These samples were taken from what will be referred to as the 'main study'. A further study was carried out to obtain samples after a partial depopulation which took place on this farm. Faecal samples were obtained from the sow housing barn, pig growing houses and slurry tanks and will be referred to as the 'depopulation (depop) study'. For the main study, the samples were collected between October 19th 2016 and April 5th 2017. For the depop study, the samples were collected between 19th June 2017 and 13th November 2017. The data associated with all samples were generated between August 1st 2017 and May 1st 2018. Full details about this dataset can be found at https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be

Simple

Date (Publication)
2018-08-10
Identifier
https://catalogue.ceh.ac.uk/id/e548dc5d-49e3-467d-9435-c199da40e7be
Identifier
doi: / 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
Other citation details
Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
Point of contact
  The Roslin Institute - Pollock, J.
Author
  SRUC/The Roslin Institute - Pollock, J.
https://orcid.org/0000-0002-2260-592X
Author
  R(D)SVS/The Roslin Institute - Corbishley, A.
Author
  SRUC - Hutchings, M.R.
Author
  The Roslin Institute - Gally, D.L.
Publisher
  NERC Environmental Information Data Centre
Custodian
  NERC EDS Environmental Information Data Centre
Owner
  University of Edinburgh
GEMET - INSPIRE themes, version 1.0
  • Soil
  • Agricultural and Aquaculture Facilities
  • Human Health and Safety
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
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This resource is made available under the terms of the Open Government Licence
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otherRestrictions Other restrictions
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© University of Edinburgh
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be
Spatial representation type
textTable Text, table
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Biota
  • Farming
Begin date
2016-10-19
End date
2017-04-05
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Unique resource identifier
OSGB 1936 / British National Grid
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
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dataset Dataset
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dataset

Conformance result

Date (Publication)
2010-12-08
Statement
Faecal and soil samples were collected from the study farm weekly using spooned universal tubes and were stored at -20 deg C, batch transported on dry ice to the laboratory where they were stored at -80 deg C until DNA extraction was carried out. DNA extracts were quantified and assessed using a NanoDrop spectrophotometer and split into aliquots for storage. DNA extracts were then screened in triplicate by qPCR and the number of gene copies assessed through the use of a standard curve generated by the inclusion of standards with known copy numbers of each target gene. Non-template controls were also included to ensure that non-specific binding or contamination were not occurring. The qPCR machine software (i.e. MxPro) was then used to calculate the number of gene copies in each of the collected samples and the mean was calculated using the three replicates. These values are included in the data set provided.
File identifier
e548dc5d-49e3-467d-9435-c199da40e7be XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
dataset Dataset
Hierarchy level name
dataset
Date stamp
2025-11-13T16:17:20
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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Keywords

GEMET - INSPIRE themes, version 1.0
Agricultural and Aquaculture Facilities Human Health and Safety Soil

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