Date (Publication)

2022-08-31

Identifier

https://catalogue.ceh.ac.uk/id/d6c787ec-146c-461b-b8a2-e0251259036c

Identifier

doi: / 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

Other citation details

Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

GEMET - INSPIRE themes, version 1.0

• Environmental Monitoring Facilities

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This resource is available under the terms of the Open Government Licence

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If you reuse this data, you should cite: Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

Spatial representation type

textTable Text, table

Distance

3  urn:ogc:def:uom:EPSG::9001

Metadata language

EnglishEnglish

Character set

utf8 UTF8

Topic category

• Biota
• Environment

Begin date

2017-08-03

End date

2019-08-05

Unique resource identifier

WGS 84 / Pseudo-Mercator

Distribution format

• Comma-separated values (CSV) ()

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dataset

Statement

The organic soil horizon (O horizon) was sampled on 3rd August 2017, 1st August 2018 and 5th August 2019. For each plot (22 in total), nine 3.8 cm diameter soil cores were collected; in the birch plots, cores were taken in a grid across a 3 x 3 m central area (corresponding to the central area in which soil CO2 efflux, root and mycelium production and soil fauna measurements were taken; (Parker et al., 2020). In the willow plots, the nine cores were distributed evenly across the plot area within the trenched perimeter. The uppermost litter layer, as well as the mineral horizons underlying the organic horizon (clearly identifiable at these sites), were removed from cores immediately after coring. The nine cores from each plot were homogenised and pooled within 6 hours of sampling. DNA was extracted from a 50 mg sub-sample and ITS2 markers were amplified by PCR. We used a community metabarcoding approach on the pooled DNA samples to identify distinct taxonomic groups at species level where possible. Copy numbers of the fungal ITS2 region were estimated by quantitative PCR (qPCR)

File identifier

d6c787ec-146c-461b-b8a2-e0251259036c XML

Metadata language

EnglishEnglish

Character set

ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1

Hierarchy level

dataset Dataset

Hierarchy level name

dataset

Date stamp

2025-03-24T09:12:00

Metadata standard name

UK GEMINI

Metadata standard version

2.3