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Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019

Fungal community DNA was sequenced from soils in northern, subarctic Sweden. Soils were sampled from treeline mountain birch forest and stands of tundra willow that were wither ‘girdled’ (connection between leaves and roots cut by disrupting the phloem transport) or ‘control’ (no treatment), 0, 1 and 2 years after the treatment was applied. Full details about this dataset can be found at https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c

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Date (Publication)
2022-08-31
Identifier
https://catalogue.ceh.ac.uk/id/d6c787ec-146c-461b-b8a2-e0251259036c
Identifier
doi: / 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c
Other citation details
Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre 10.5285/d6c787ec-146c-461b-b8a2-e0251259036c
Author
  James Hutton Institute - Parker, T.C.
https://orcid.org/0000-0002-3648-5316
Author
  University of Manchester - Chomel, M.
https://orcid.org/0000-0001-5110-2355
Author
  Swedish University of Agricultural Sciences - Clemmensen, K.E.
https://orcid.org/0000-0002-9627-6428
Author
  University of Exeter - Friggens, N.L.
https://orcid.org/0000-0002-5536-6312
Author
  University of Exeter - Hartley, I.P.
https://orcid.org/0000-0002-9183-6617
Author
  University of Manchester - Johnson, D.
https://orcid.org/0000-0003-2299-2525
Author
  Swedish University of Agricultural Sciences - Krab, E.J.
https://orcid.org/0000-0001-8262-0198
Author
  Swedish University of Agricultural Sciences - Lindahl, B.D.
https://orcid.org/0000-0002-3384-4547
Author
  University of Edinburgh - Street, L.E.
https://orcid.org/0000-0001-9570-7479
Author
  University of Stirling - Subke, J.-.
https://orcid.org/0000-0001-9244-639X
Author
  University of Stirling - Wookey, P.A.
https://orcid.org/0000-0001-5957-6424
Point of contact
  James Hutton Institute - Parker, T.
Custodian
  NERC EDS Environmental Information Data Centre
Owner
  University of Stirling
Publisher
  NERC EDS Environmental Information Data Centre
GEMET - INSPIRE themes, version 1.0
  • Environmental Monitoring Facilities
Access constraints
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no limitations
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otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Parker, T.C., Chomel, M., Clemmensen, K.E., Friggens, N.L., Hartley, I.P., Johnson, D., Krab, E.J., Lindahl, B.D., Street, L.E., Subke, J.-., Wookey, P.A. (2022). Soil fungi gene copy numbers and community composition under birch and willow vegetation in girdled and non-girdled plots, subarctic Sweden, 2017-2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/d6c787ec-146c-461b-b8a2-e0251259036c
Spatial representation type
textTable Text, table
Distance
3  urn:ogc:def:uom:EPSG::9001
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Biota
  • Environment
Begin date
2017-08-03
End date
2019-08-05
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Unique resource identifier
WGS 84 / Pseudo-Mercator
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
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Conformance result

Date (Publication)
2010-12-08
Statement
The organic soil horizon (O horizon) was sampled on 3rd August 2017, 1st August 2018 and 5th August 2019. For each plot (22 in total), nine 3.8 cm diameter soil cores were collected; in the birch plots, cores were taken in a grid across a 3 x 3 m central area (corresponding to the central area in which soil CO2 efflux, root and mycelium production and soil fauna measurements were taken; (Parker et al., 2020). In the willow plots, the nine cores were distributed evenly across the plot area within the trenched perimeter. The uppermost litter layer, as well as the mineral horizons underlying the organic horizon (clearly identifiable at these sites), were removed from cores immediately after coring. The nine cores from each plot were homogenised and pooled within 6 hours of sampling. DNA was extracted from a 50 mg sub-sample and ITS2 markers were amplified by PCR. We used a community metabarcoding approach on the pooled DNA samples to identify distinct taxonomic groups at species level where possible. Copy numbers of the fungal ITS2 region were estimated by quantitative PCR (qPCR)
File identifier
d6c787ec-146c-461b-b8a2-e0251259036c XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
dataset Dataset
Hierarchy level name
dataset
Date stamp
2025-03-24T09:12:00
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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GEMET - INSPIRE themes, version 1.0
Environmental Monitoring Facilities

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