NERC Data Catalogue Service
Weight loss assay data of Polycaprolactone beads exposed to genetically modified microbes expressing plastic degrading enzymes under laboratory conditions
[This nonGeographicDataset is embargoed until June 4, 2026]. This dataset contains information about the weight of plastic (polycaprolactone) beads that were incubated in 10ml of Luria Bertani broth for 5 days at 37 degrees Celsius. Three replicates were performed. The bacterial cells had been modified to harbour plasmids that encoded genes to degrade plastic and/or to trigger the cells to form biofilms, the expression of these genes was induced using Isopropyl β-d-1-thiogalactopyranoside (IPTG). An empty plasmid was used as the control. The work was supported by the Natural Environment Research Council (Grant NE/X010902/1), as part of an experiment looking into using synthetic biology to manipulate bacterial social behaviours to maximise the microbial degradation of environmental waste plastics. Full details about this nonGeographicDataset can be found at
https://doi.org/10.5285/bd582f82-ba16-4bc6-8a25-ec29bb22bb5b
Simple
- Date (Publication)
- 2025-06-30
- Identifier
- doi: / 10.5285/bd582f82-ba16-4bc6-8a25-ec29bb22bb5b
- Other citation details
- McCarthy, R., Howard, S. (2025). Weight loss assay data of Polycaprolactone beads exposed to genetically modified microbes expressing plastic degrading enzymes under laboratory conditions. NERC EDS Environmental Information Data Centre 10.5285/bd582f82-ba16-4bc6-8a25-ec29bb22bb5b
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- Embargoed
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- Creative Commons Attribution 4.0 International (CC BY 4.0)
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: McCarthy, R., Howard, S. (2025). Weight loss assay data of Polycaprolactone beads exposed to genetically modified microbes expressing plastic degrading enzymes under laboratory conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/bd582f82-ba16-4bc6-8a25-ec29bb22bb5b
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
-
- Environment
- Distribution format
-
-
Comma-separated values (CSV)
()
-
Comma-separated values (CSV)
()
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Other
- nonGeographicDataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- For Polycaprolactone (PCL) weight loss assays, overnight cultures grown in Luria Bertani (LB) broth at 37 degrees C were used to inoculate 10 mL fresh LB at OD600 0.1 containing sterilised PCL beads. The cultures were grown at 37 degrees C with agitation until OD600 0.6, then were induced with 1 mM IPTG and grown for 5 days. The culture media was refreshed after 3 days due to Ampicillin instability (culture collected, centrifuged at 5000 × g for 15 minutes, pellet resuspended in fresh media and returned to flask). The PCL beads were collected using a fine mesh sieve (63 um), rinsed with distilled water, washed on a room temperature rocker in 2% sodium dodecyl sulphate overnight to remove biofilm, rinsed with distilled water and dried before weighing. Control weight loss flasks with uninoculated media and PCL were used to correct the weight loss measurements to account for instrument error or nonbiological degradation from spontaneous hydrolysis so that the control had 0% weight loss.
- File identifier
- bd582f82-ba16-4bc6-8a25-ec29bb22bb5b XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Hierarchy level name
- nonGeographicDataset
- Date stamp
- 2025-08-04T10:27:47
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
