Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves
This dataset contains experimental survival rate and transfer potential measurements of two known human pathogens, bacteria Salmonella Typhimurium and Vibrio cholerae, on plastic mulch and ready-to-eat crop plants. Colony forming units (CFUs) were used to measure persistence time of each pathogen on the mulch, and quantify the transfer of each pathogen from the plastic mulch to the surface of spinach and basil leaves. Samples were measured on days 1, 2, 3, 4, 7, 10 and 14, over a total period of 14 days. Destructive sampling methods were used to collect samples from the plastic and leaf surface, with negative control leaves also sampled at each time point. This dataset was created as part of a study investigating the risk of pathogen growth in agricultural environments using plastic mulch, and is supported by the National Environmental Research Council (NE/V005847/1). Full details about this nonGeographicDataset can be found at
https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
Simple
- Date (Publication)
- 2025-05-02
- Identifier
- doi: / 10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
- Other citation details
- Woodford, L., White, H., Fellows, R., Ormsby, M., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves. NERC EDS Environmental Information Data Centre 10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
- GEMET - INSPIRE themes, version 1.0
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- Human Health and Safety
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions Other restrictions
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: Woodford, L., White, H., Fellows, R., Ormsby, M., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
-
- Environment
- Begin date
- 2023-10-01
- End date
- 2023-11-01
- Distribution format
-
-
Comma-separated values (CSV)
()
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Comma-separated values (CSV)
()
- OnLine resource
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Download the data
Download a copy of this data
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Supporting information
Supporting information available to assist in re-use of this dataset
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Other
- nonGeographicDataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- Persistence experiments, examining the survival of S. Typhimurium and V. cholerae were set up by placing spinach and basil leaves in petri dishes and adding plastic mulch with a biofilm containing each pathogen to the leaf surface. Over time samples were destructively sampled by removing the plastic, washing the leaf surface and cutting a circle from the leaf. These three sections (plastic, wash and cut leaf) were then resuspended and plated on to selevctive media. Any surviving bacteria were quantified using CFUs. Negative control leaves with no plastics or bacteria were analysed at each time point.
- File identifier
- a514a386-18d7-4925-a3d4-fb5a2922e135 XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Hierarchy level name
- nonGeographicDataset
- Date stamp
- 2025-11-13T16:18:54
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
NERC Data Catalogue Service