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Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves

This dataset contains experimental survival rate and transfer potential measurements of two known human pathogens, bacteria Salmonella Typhimurium and Vibrio cholerae, on plastic mulch and ready-to-eat crop plants. Colony forming units (CFUs) were used to measure persistence time of each pathogen on the mulch, and quantify the transfer of each pathogen from the plastic mulch to the surface of spinach and basil leaves. Samples were measured on days 1, 2, 3, 4, 7, 10 and 14, over a total period of 14 days. Destructive sampling methods were used to collect samples from the plastic and leaf surface, with negative control leaves also sampled at each time point. This dataset was created as part of a study investigating the risk of pathogen growth in agricultural environments using plastic mulch, and is supported by the National Environmental Research Council (NE/V005847/1). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135

Simple

Date (Publication)
2025-05-02
Identifier
https://catalogue.ceh.ac.uk/id/a514a386-18d7-4925-a3d4-fb5a2922e135
Identifier
doi: / 10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
Other citation details
Woodford, L., White, H., Fellows, R., Ormsby, M., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves. NERC EDS Environmental Information Data Centre 10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
Point of contact
  University of Stirling - Woodford, L.
Author
  University of Stirling - Woodford, L.
https://orcid.org/0000-0003-2530-2120
Author
  University of Stirling - White, H.
Author
  University of Stirling - Fellows, R.
Author
  University of Stirling - Ormsby, M.
Author
  University of Stirling - Quilliam, R.
Owner
  University of Stirling
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC EDS Environmental Information Data Centre
GEMET - INSPIRE themes, version 1.0
  • Human Health and Safety
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Woodford, L., White, H., Fellows, R., Ormsby, M., Quilliam, R. (2025). Survival rates and transfer potential measurements of Salmonella Typhimurium and Vibrio cholerae colonising plastic mulch onto basil and spinach salad leaves. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/a514a386-18d7-4925-a3d4-fb5a2922e135
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Environment
Begin date
2023-10-01
End date
2023-11-01
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
Persistence experiments, examining the survival of S. Typhimurium and V. cholerae were set up by placing spinach and basil leaves in petri dishes and adding plastic mulch with a biofilm containing each pathogen to the leaf surface. Over time samples were destructively sampled by removing the plastic, washing the leaf surface and cutting a circle from the leaf. These three sections (plastic, wash and cut leaf) were then resuspended and plated on to selevctive media. Any surviving bacteria were quantified using CFUs. Negative control leaves with no plastics or bacteria were analysed at each time point.
File identifier
a514a386-18d7-4925-a3d4-fb5a2922e135 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-11-13T16:18:54
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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Keywords

GEMET - INSPIRE themes, version 1.0
Human Health and Safety

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