The resource includes data extracted from published laboratory studies investigating the impacts of microfibres (fibres < 5 mm in size) on various traits within aquatic organisms. Data were accessible on 19/06/2024 and represent studies from 2015 to 2024. The dataset includes measured responses in a range of aquatic organisms after microfibre exposure. Measures correspond to the following defined traits: 1. Development-Healthy molting and growth of organs and structures. 2. Fecundity-Number of offspring and quality of gametes. 3. Feeding-Intake, handling and egestion of resources. 4. Growth-Change in organism weight, length or derived measures. 5. Hatching -Number, emergence time and morphology of offspring. 6. Non-apical endpoints-Indices and measures of general organism wellbeing. 7. Survival-Organisms alive at a particular time point. This dataset was created to collate microfibre study findings for meta-analyses and also to identify gaps within the literature. This work was supported by NERC (NE/Y003985/1) as part of the Future Fibres Networking Grant. This dataset is a corrected version of https://doi.org/10.5285/e4d566bf-b571-460e-816e-0efa1ab3335a Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/8b7fcc6c-ac73-4f28-b82f-ea818b064915