NERC Data Catalogue Service
Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD)
[This dataset is embargoed until October 28, 2025]. This dataset contains information about the development of a paper analytical device for the detection of the microcystin toxin. Water samples were collected weekly from Rostherne Mere and Tatton Park Lake, Cheshire between 20/07/2022 and 12/10/2022. Samples were analysed for the presence of microcystin-producing genes and released microcystin. A paper analytical device incorporating a protein phosphatase inhibition assay was also used to monitor microcystin levels. Preliminary user evaluation of the paper analytical devices and associated mobile photo applications is also provided. The work was supported by the Natural Environment Research Council (Grant NE/X011607/1). Full details about this dataset can be found at
https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
Simple
- Date (Publication)
- 2024-06-12
- Identifier
- doi: / 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
- Other citation details
- Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
- GEMET - INSPIRE themes, version 1.0
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- Environmental Monitoring Facilities
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- Embargoed
- Use constraints
- otherRestrictions Other restrictions
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
- Spatial representation type
- textTable Text, table
- Distance
- 1000 urn:ogc:def:uom:EPSG::9001
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
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- Environment
- Begin date
- 2022-06-20
- End date
- 2022-10-12
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W
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- Unique resource identifier
- OSGB 1936 / British National Grid
- Distribution format
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Comma-separated values (CSV)
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jpeg
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Comma-separated values (CSV)
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- Hierarchy level
- dataset Dataset
- Other
- dataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- Water samples were taken on a weekly basis from three sampling locations at each site. Samples were filtered through a 0.45 µm filter and DNA was extracted from the filter paper using a Qiagen PowerWater kit before being analysed using real-time PCR for detection of the mycE gene, alongside no template controls. Water samples were analysed using an ENZO Life Sciences Microcystins (Adda specific) ELISA kit, alongside microcystin reference standards and controls. For the development of the paper analytical device, protein phosphatase 1a was produced using protein expression and confirmed using spectrophotometry. Efficiency was evaluated by comparison to commercially available products and comparing absorbance values for the conversion of pNPP. The paper analytical device incorporating the protein phosphatase inhibition assay was then used to detect microcystin. Colourimetric change was recorded for samples and controls using Image J. Preliminary user evaluation of the paper analytical device was obtained using qualitative feedback.
- File identifier
- 5fe25b28-f10b-467b-8812-0b1ee6fd7491 XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- dataset Dataset
- Hierarchy level name
- dataset
- Date stamp
- 2025-03-21T13:22:04
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
Overviews
Spatial extent
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Associated resources
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