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Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD)

[This dataset is embargoed until October 28, 2025]. This dataset contains information about the development of a paper analytical device for the detection of the microcystin toxin. Water samples were collected weekly from Rostherne Mere and Tatton Park Lake, Cheshire between 20/07/2022 and 12/10/2022. Samples were analysed for the presence of microcystin-producing genes and released microcystin. A paper analytical device incorporating a protein phosphatase inhibition assay was also used to monitor microcystin levels. Preliminary user evaluation of the paper analytical devices and associated mobile photo applications is also provided. The work was supported by the Natural Environment Research Council (Grant NE/X011607/1). Full details about this dataset can be found at https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491

Simple

Date (Publication)
2024-06-12
Identifier
https://catalogue.ceh.ac.uk/id/5fe25b28-f10b-467b-8812-0b1ee6fd7491
Identifier
doi: / 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
Other citation details
Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre 10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
Author
  Manchester Metropolitan University - Hill, M.
Author
  Manchester Metropolitan University - Jenkinson, L.A.
Author
  Manchester Metropolitan University - Dean, A.P.
https://orcid.org/0000-0001-6893-5118
Author
  Manchester Metropolitan University - Redfern, J.
https://orcid.org/0000-0003-0958-683X
Author
  Manchester Metropolitan University - Shaw, K.J.
https://orcid.org/0000-0001-9241-4195
Point of contact
  Manchester Metropolitan University - Shaw, K.
Publisher
  NERC EDS Environmental Information Data Centre
Custodian
  NERC EDS Environmental Information Data Centre
Owner
  Manchester Metropolitan University
GEMET - INSPIRE themes, version 1.0
  • Environmental Monitoring Facilities
Access constraints
otherRestrictions Other restrictions
Other constraints
Embargoed
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Hill, M., Jenkinson, L.A., Dean, A.P., Redfern, J., Shaw, K.J. (2024). Quantitative and qualitative data relating to the inhibition of a phosphatase reaction by microcystin on a paper-based analytical device (PAD). NERC EDS Environmental Information Data Centre https://doi.org/10.5285/5fe25b28-f10b-467b-8812-0b1ee6fd7491
Spatial representation type
textTable Text, table
Distance
1000  urn:ogc:def:uom:EPSG::9001
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Environment
Begin date
2022-06-20
End date
2022-10-12
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Unique resource identifier
OSGB 1936 / British National Grid
Distribution format
  • Comma-separated values (CSV) ()

  • jpeg ()

Distributor
  NERC EDS Environmental Information Data Centre
Hierarchy level
dataset Dataset
Other
dataset

Conformance result

Date (Publication)
2010-12-08
Statement
Water samples were taken on a weekly basis from three sampling locations at each site. Samples were filtered through a 0.45 µm filter and DNA was extracted from the filter paper using a Qiagen PowerWater kit before being analysed using real-time PCR for detection of the mycE gene, alongside no template controls. Water samples were analysed using an ENZO Life Sciences Microcystins (Adda specific) ELISA kit, alongside microcystin reference standards and controls. For the development of the paper analytical device, protein phosphatase 1a was produced using protein expression and confirmed using spectrophotometry. Efficiency was evaluated by comparison to commercially available products and comparing absorbance values for the conversion of pNPP. The paper analytical device incorporating the protein phosphatase inhibition assay was then used to detect microcystin. Colourimetric change was recorded for samples and controls using Image J. Preliminary user evaluation of the paper analytical device was obtained using qualitative feedback.
File identifier
5fe25b28-f10b-467b-8812-0b1ee6fd7491 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
dataset Dataset
Hierarchy level name
dataset
Date stamp
2025-03-21T13:22:04
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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Keywords

GEMET - INSPIRE themes, version 1.0
Environmental Monitoring Facilities

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