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Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA

This dataset contains untargeted metabolomic profiles of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458). Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e

Simple

Date (Publication)
2025-02-12
Identifier
https://catalogue.ceh.ac.uk/id/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
Identifier
doi: / 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
Other citation details
Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
Author
  University of Manchester - Connolly, J.A.
https://orcid.org/0000-0003-3643-5578
Author
  University of Manchester - Del Carratore, F.
https://orcid.org/0000-0003-1647-7818
Author
  University of Manchester - Schmidt, K.
Author
  University of Minnesota - Bisesi, A.
Author
  University of Minnesota - Martinson, J.N.V.
Author
  University of Manchester - Chua, J.
Author
  University of Minnesota - Kuhs, M.
Author
  University of Minnesota - Boneza, M.
Author
  University of Minnesota - Heinsch, S.
Author
  University of Minnesota - Kinkel, L.
https://orcid.org/0000-0002-5331-5589
Author
  University of Minnesota - Smanski, M.
Author
  University of Minnesota - Harcombe, W.R.
Author
  University of Manchester - Breitling, R.
https://orcid.org/0000-0001-7173-0922
Author
  University of Manchester - Takano, E.
https://orcid.org/0000-0002-6791-3256
Owner
  University of Manchester
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC EDS Environmental Information Data Centre
Point of contact
  University of Manchester - Connolly, J.A.
GEMET - INSPIRE themes, version 1.0
  • Species Distribution
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Biota
Distribution format
  • .mzML ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.
File identifier
475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-06-03T07:41:51
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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GEMET - INSPIRE themes, version 1.0
Species Distribution

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