NERC Data Catalogue Service
Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA
This dataset contains untargeted metabolomic profiles of paired cultures of 4 interacting Streptomyces bacteria. The bacteria were originally isolated from a nature reserve in Minnesota USA. Daily samples of spot cultures grown in adjacent pairs on solid ISP2 medium were taken from day 2 to 6 in triplicate. Metabolites were obtained by cold methanol extraction, and liquid chromatography-mass spectrometry data was collected in both positive and negative ionisation modes. This dataset contains raw LC-MS (liquid chromatography mass spectrometry) data converted to the open-source .mzML format, of each sample run in both positive and negative ionisation modes. This includes, mass-to-charge measurements, elution time, and ion count intensity. These data were collected to investigate how the metabolism of the interacting strains changes in response to neighbouring bacterial cultures. This work was funded by NERC (NE/T010959/1) and National Science Foundation (USA, 1935458). Full details about this nonGeographicDataset can be found at
https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
Simple
- Date (Publication)
- 2025-02-12
- Identifier
- doi: / 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
- Other citation details
- Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre 10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
- GEMET - INSPIRE themes, version 1.0
-
- Species Distribution
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions Other restrictions
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: Connolly, J.A., Del Carratore, F., Schmidt, K., Bisesi, A., Martinson, J.N.V., Chua, J., Kuhs, M., Boneza, M., Heinsch, S., Kinkel, L., Smanski, M., Harcombe, W.R., Breitling, R., Takano, E. (2025). Untargeted metabolomic profiles of paired cultures of four strains of Streptomyces bacteria isolated from a nature reserve in Minnesota, USA. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
-
- Biota
- Distribution format
-
-
.mzML
()
-
.mzML
()
- OnLine resource
-
Download the data
Download a copy of this data
- OnLine resource
-
Supporting information
Supporting information available to assist in re-use of this dataset
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Other
- nonGeographicDataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- The 4 Streptomyces strains were previously isolated from Minnesota soil as described in Schlatter, D.C. et al (2013), co-culturing, metabolite extraction and LC-MS experiments were performed in the Manchester Institute of Biotechnology, University of Manchester, UK. Data was collected on a Q-Exactive Mass Spectrometer (ThermoFisher). Outputs were converted to mzML format by the ProteoWizard MS converter. Pooled quality control samples were analysed at every 6th injection to assess for analytical drift over time. The sample injection sequence was randomised.
- File identifier
- 475d6c02-45bb-4ac4-a31a-4a23c7c9cb2e XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Hierarchy level name
- nonGeographicDataset
- Date stamp
- 2025-06-03T07:41:51
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
