NERC Data Catalogue Service
Drosophila C virus genomes, Cambridge (2017)
The resource consists of genome sequence data for the Drosophila C virus that has been serially passaged through different species of Drosophila in the laboratory. The genomes were sequenced and aligned to the reference genome. The frequency of variants at both biallelic and triallelic sites was then calculated. We also generated a phylogeny of the species involved using published data. This data was generated to understand how viruses adapt to new host species by Francis Jiggins and his co workers. The work was carried out between July 2016 and September 2017 and was funded by NERC under award reference NE/L004232/1 Full details about this nonGeographicDataset can be found at
https://doi.org/10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073
Simple
- Date (Publication)
- 2018-01-24
- Identifier
- doi: / 10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073
- Other citation details
- Jiggins, F.M., Longdon, B. (2018). Drosophila C virus genomes, Cambridge (2017). NERC Environmental Information Data Centre 10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073
- Maintenance and update frequency
- notPlanned Not planned
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions Other restrictions
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- © Cambridge University
- Use constraints
- otherRestrictions Other restrictions
- Other constraints
- If you reuse this data, you should cite: Jiggins, F.M., Longdon, B. (2018). Drosophila C virus genomes, Cambridge (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
-
- Health
- Begin date
- 2016-01-01
- End date
- 2017-09-01
- Distribution format
-
-
Comma-separated values (CSV)
()
-
BAM
()
-
Comma-separated values (CSV)
()
- OnLine resource
-
Supporting information
Supporting information available to assist in re-use of this dataset
- OnLine resource
-
Download the data
Download a copy of this data
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Other
- nonGeographicDataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- Each species of Drosophila was infected by dipping a needle in virus suspension. After 3 days the fly was then homogenized and the process repeated for 8-10 passages. At the end of this process, Ribonucleic acid (RNA) was extracted, the viral genome amplified by Polymerase chain reaction (PCR) and Nextera libraries constructed. These were sequenced on n Illumina sequencer, the sequences quality trimmed and aligned to the reference genome to generate the BAM file in this dataset. We then called variants to generate the allele frequency CSV files. Full details of these steps are provided in the supporting documentation. NB: these data are not adequately described in that there are no column descriptions for the csv files.
- File identifier
- 4434a27d-5288-4f2e-88ac-4b1372e4d073 XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- nonGeographicDataset Non geographic dataset
- Hierarchy level name
- nonGeographicDataset
- Date stamp
- 2025-11-13T16:15:44
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
