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Drosophila C virus genomes, Cambridge (2017)

The resource consists of genome sequence data for the Drosophila C virus that has been serially passaged through different species of Drosophila in the laboratory. The genomes were sequenced and aligned to the reference genome. The frequency of variants at both biallelic and triallelic sites was then calculated. We also generated a phylogeny of the species involved using published data. This data was generated to understand how viruses adapt to new host species by Francis Jiggins and his co workers. The work was carried out between July 2016 and September 2017 and was funded by NERC under award reference NE/L004232/1 Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073

Simple

Date (Publication)
2018-01-24
Identifier
https://catalogue.ceh.ac.uk/id/4434a27d-5288-4f2e-88ac-4b1372e4d073
Identifier
doi: / 10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073
Other citation details
Jiggins, F.M., Longdon, B. (2018). Drosophila C virus genomes, Cambridge (2017). NERC Environmental Information Data Centre 10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073
Point of contact
  University of Cambridge - Jiggins, F.
Author
  University of Cambridge - Jiggins, F.M.
https://orcid.org/0000-0001-7470-8157
Author
  University of Exeter - Longdon, B.
https://orcid.org/0000-0001-6936-1697
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC Environmental Information Data Centre
Owner
  University of Cambridge
Maintenance and update frequency
notPlanned Not planned
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is made available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
© Cambridge University
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Jiggins, F.M., Longdon, B. (2018). Drosophila C virus genomes, Cambridge (2017). NERC Environmental Information Data Centre https://doi.org/10.5285/4434a27d-5288-4f2e-88ac-4b1372e4d073
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Health
Begin date
2016-01-01
End date
2017-09-01
Distribution format
  • Comma-separated values (CSV) ()

  • BAM ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
Supporting information

Supporting information available to assist in re-use of this dataset

OnLine resource
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
Each species of Drosophila was infected by dipping a needle in virus suspension. After 3 days the fly was then homogenized and the process repeated for 8-10 passages. At the end of this process, Ribonucleic acid (RNA) was extracted, the viral genome amplified by Polymerase chain reaction (PCR) and Nextera libraries constructed. These were sequenced on n Illumina sequencer, the sequences quality trimmed and aligned to the reference genome to generate the BAM file in this dataset. We then called variants to generate the allele frequency CSV files. Full details of these steps are provided in the supporting documentation. NB: these data are not adequately described in that there are no column descriptions for the csv files.
File identifier
4434a27d-5288-4f2e-88ac-4b1372e4d073 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-11-13T16:15:44
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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