• NERC Data Catalogue Service
  •  
  •  
  •  

River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB

The data are from bioassay experiments from 25 river sites across Great Britain to investigate the growth response of river phytoplankton to different inorganic and organic nutrient additions and compare that to in situ nutrient concentrations. The data includes calculated growth responses to inorganic and organic nutrient additions, based on experimental incubations and background nutrient data collected from sites at the same time as water for the experimental incubations. Full details about this dataset can be found at https://doi.org/10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a

Simple

Date (Publication)
2025-03-27
Identifier
https://catalogue.ceh.ac.uk/id/3ca94e73-57dc-4fcd-afe2-82a4e491970a
Identifier
doi: / 10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a
Other citation details
Mackay, E.B., Feuchtmayr, H., Thackeray, S.J., Callaghan, N., Marshall, M., Rhodes, G., Yates, C., Johnes, P.J., Maberly, S.C. (2025). River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB. NERC EDS Environmental Information Data Centre 10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a
Author
  UK Centre for Ecology & Hydrology - Mackay, E.B.
https://orcid.org/0000-0001-5697-7062
Author
  UK Centre for Ecology & Hydrology - Feuchtmayr, H.
Author
  UK Centre for Ecology & Hydrology - Thackeray, S.J.
Author
  UK Centre for Ecology & Hydrology - Callaghan, N.
Author
  Bangor University - Marshall, M.
Author
  UK Centre for Ecology & Hydrology - Rhodes, G.
Author
  Atkins Realis - Yates, C.
Author
  Bristol University - Johnes, P.J.
Author
  UK Centre for Ecology & Hydrology - Maberly, S.C.
Point of contact
  UK Centre for Ecology & Hydrology - Mackay, E.B.
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC EDS Environmental Information Data Centre
Owner
  UK Centre for Ecology & Hydrology
Maintenance and update frequency
notPlanned Not planned
GEMET - INSPIRE themes, version 1.0
  • Environmental Monitoring Facilities
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Mackay, E.B., Feuchtmayr, H., Thackeray, S.J., Callaghan, N., Marshall, M., Rhodes, G., Yates, C., Johnes, P.J., Maberly, S.C. (2025). River phytoplankton growth responses to organic nutrients with associated nutrient data from 25 rivers in GB. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/3ca94e73-57dc-4fcd-afe2-82a4e491970a
Spatial representation type
textTable Text, table
Distance
100  urn:ogc:def:uom:EPSG::9001
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Environment
Begin date
2017-09-05
End date
2017-10-26
N
S
E
W
thumbnail


Unique resource identifier
OSGB 1936 / British National Grid
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
Download the data

Download a copy of this data

OnLine resource
Supporting information

Supporting information available to assist in re-use of this dataset

Hierarchy level
dataset Dataset
Other
dataset

Conformance result

Date (Publication)
2010-12-08
Statement
Raw water samples were collected at each site on a single sampling occasion and kept at 4°C in the dark and transported to the laboratory within 48 hours. They were acclimatised in a constant temperature room at 20°C. Water was filtered through a 100 µm mesh to remove any zooplankton or large detritus present, 35 mL of sample was transferred to 50 mL boiling tubes. 12 different nutrient treatments were applied at approximately Redfield ratio [N: 90 µmol L-1 (1.261 mg N L-1) and P: 6 µmol L-1 (0.186 mg P L-1)] in triplicate to quantify growth responses to both organic N and P. These included: 1) a control with no addition of nutrients, 2) inorganic N only, 3) inorganic P only and 4) inorganic N and P, 5-8) four organic N additions and 9- 12) four organic P additions. Tubes were stoppered with foam bungs enabling gas exchange and incubated for 14 days at 20°C under a photon irradiance of 80 – 120 µmol m-2 s-1 (photosynthetically available radiation, Macam Q102) and an 18 h light, 6 h dark cycle. After the incubation period, tube contents including biofilm were resuspended by scraping and vortex mixing before being filtered onto a Whatman GF/C glass fibre filter (nominal pore size 1.2 µm) and frozen. Filters were later defrosted, and chlorophyll a extracted in hot methanol and measured spectrophotometrically. Growth response was determined as a change in the concentration of chlorophyll a in the treatment relative to the relevant control calculated as a natural log response ratio. For the water chemistry data (measured in mg/L), a Skalar++ multi-channel continuous flow autoanalyzer (Skalar Analytical B.V., The Netherlands) was used to carry out inorganic nutrient analysis, including total and total dissolved N and P (TN or TP, TDN or TDP) following persulphate digestion of unfiltered and filtered (pre-leached 0.45 µm cellulose nitrate) samples respectively, and dissolved inorganic N (DIN), including total ammoniacal nitrogen (NH3 and NH4), or soluble reactive P (SRP) on filtered samples. Dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP), were determined by difference between TDN and DIN or TDP and SRP, respectively. Data from these analyses were checked and entered into spreadsheets for later analysis.
File identifier
3ca94e73-57dc-4fcd-afe2-82a4e491970a XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
dataset Dataset
Hierarchy level name
dataset
Date stamp
2025-03-31T12:55:49
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

Overviews

Spatial extent

N
S
E
W
thumbnail


Keywords

GEMET - INSPIRE themes, version 1.0
Environmental Monitoring Facilities

Provided by

logo

Share on social sites

Access to the portal
Read here the full details and access to the data.

Associated resources

Not available


  •  
  •  
  •