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Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013)

The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems. The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme. Full details about this dataset can be found at https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274

Simple

Date (Publication)
2017-09-20
Date (Creation)
2013-06-15
Identifier
https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274
Identifier
doi: / 10.5285/27da4400-9835-4241-9df9-a3b598f8d274
Other citation details
MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre 10.5285/27da4400-9835-4241-9df9-a3b598f8d274
Point of contact
  Cardiff University - MacDonald, H.
Author
  Cardiff University - MacDonald, H.C.
Author
  Cardiff University - Bruford, M.W.
Author
  Cardiff University - Ormerod, S.
https://orcid.org/0000-0002-8174-302X
Author
  Cardiff University - Jones, T.H.
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC Environmental Information Data Centre
Owner
  Cardiff University
Maintenance and update frequency
notPlanned Not planned
GEMET - INSPIRE themes, version 1.0
  • Habitats and Biotopes
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is made available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
© Cardiff University
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: MacDonald, H.C., Bruford, M.W., Ormerod, S., Jones, T.H. (2017). Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013). NERC Environmental Information Data Centre https://doi.org/10.5285/27da4400-9835-4241-9df9-a3b598f8d274
Spatial representation type
textTable Text, table
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Biota
Begin date
2012-06-15
End date
2013-06-15
N
S
E
W
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Unique resource identifier
OSGB 1936 / British National Grid
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
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Supporting information

Supporting information available to assist in re-use of this dataset

Hierarchy level
dataset Dataset
Other
dataset

Conformance result

Date (Publication)
2010-12-08
Statement
Samples of each invertebrate were collected by kicksampling in May in 2012 and 2013, samples were sorted and identified to genus level on site and stored in absolute ethanol. In the laboratory each larva was identified to species level using Elliott et al. (1988) and Hynes (1977). DNA was then extracted from tissue samples (see supporting information for extraction methods). Baetis rhodani samples contained cryptic species which had to be removed before genotyping, no cryptic species were identified for Amphinemura sulcicollis or Isoperla grammatica. Samples were sent for fragment analysis (Dundee Biosciences, UK) using ROX500 as the internal size standard. Fragment analysis products were visualised and scored using Genemarker v 1.91 (Holland and Parson 2011). These results were downloaded from Genemarker and allele sizes were binned by hand using the 'Binning' information (provided in the metadata) in an Excel spreadsheet. The data were then complied into master Excel spreadsheets for each invertebrate. The data were then exported as comma separated value files for ingestion into the EIDC.
File identifier
27da4400-9835-4241-9df9-a3b598f8d274 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
dataset Dataset
Hierarchy level name
dataset
Date stamp
2025-11-05T08:34:17
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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Spatial extent

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Keywords

GEMET - INSPIRE themes, version 1.0
Habitats and Biotopes

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