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Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions

This dataset contains information relating to the survival and persistence of clinically relevant strains of E. coli on plastic and cotton, under environmentally relevant conditions. The data encompass bacterial strain identity, material type, timepoints (days), bacterial concentration (CFU/ml) and survival percentages. Data were sampled over a 28-day period using culture-based approaches; and the ability of isolates to cause disease following their recovery was determined through the use of a Galleria mellonella model. This work was supported by the UKRI Natural Environment Research Council (NERC) as part of the GCRF SPACES project [grant number NE/V005847/1] and the Plastic Vectors project, “Microbial hitch-hikers of marine plastics: the survival, persistence & ecology of microbial communities in the ‘Plastisphere’” [grant number NE/S005196/1]. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2

Simple

Date (Publication)
2025-04-09
Identifier
https://catalogue.ceh.ac.uk/id/268cab8c-459c-4bf8-9b90-838c4d18efb2
Identifier
doi: / 10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
Other citation details
Ormsby, M.J., White, H.L., Metcalf, R., Oliver, D.M., Quilliam, R.S. (2025). Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions. NERC EDS Environmental Information Data Centre 10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
Author
  University of Stirling - Ormsby, M.J.
https://orcid.org/0000-0002-3991-2336
Point of contact
  University of Stirling - Ormsby, M.J.
https://orcid.org/0000-0002-3991-2336
Author
  University of Stirling - White, H.L.
Author
  University of Stirling - Metcalf, R.
Author
  University of Stirling - Oliver, D.M.
https://orcid.org/0000-0002-6200-562X
Author
  University of Stirling - Quilliam, R.S.
https://orcid.org/0000-0001-7020-4410
Owner
  University of Stirling
Custodian
  NERC EDS Environmental Information Data Centre
Publisher
  NERC EDS Environmental Information Data Centre
GEMET - INSPIRE themes, version 1.0
  • Human Health and Safety
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Ormsby, M.J., White, H.L., Metcalf, R., Oliver, D.M., Quilliam, R.S. (2025). Survival and persistence of clinically relevant E. coli on plastic waste under simulated environmental conditions. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/268cab8c-459c-4bf8-9b90-838c4d18efb2
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Environment
Begin date
2023-01-01
End date
2023-06-28
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
Biofilm formation, persistence and pathogenicity of E. coli were investigated through controlled laboratory experiments. Biofilms were generated on various materials in nutrient-rich and surface water environments, quantified using crystal violet staining, and analysed in triplicate. Persistence was assessed by recovering E. coli from biofilms over 28 days, followed by serial dilution, and selective plating. Pathogenicity was evaluated using Galleria mellonella larvae, with standardised inoculation protocols and survival monitoring for 72 hours. Data underwent thorough validation at each stage, including replication, and selective media confirmation, ensuring high reliability and accuracy before submission.
File identifier
268cab8c-459c-4bf8-9b90-838c4d18efb2 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-04-10T15:32:18
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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Keywords

GEMET - INSPIRE themes, version 1.0
Human Health and Safety

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