This dataset contains a list of all known birds, bryophytes, fungi, invertebrates, lichens and mammals that use oak (Quercus petraea and Quercus robur) in the UK. In total 2300 species are listed in the dataset. For each species we provide a level of association with oak, ranging from obligate (only found on oak) to cosmopolitan (found on a wide range of other tree species). Data on the ecology of each oak associated species is provided: part of tree used, use made of tree (feeding, roosting, breeding), age of tree, woodland type, tree form (coppice, pollarded, or natural growth form) and season when the tree was used. Data on use or otherwise by each of the 2300 species of 30 other alternative tree species (Acer campestre, Acer pseudoplatanus, Alnus glutinosa, Betula pendula, Betula pubescens, Carpinus betulus, Castanea sativa, Fagus sylvatica, Fraxinus excelsior, Ilex aquifolium, Larix spp, Malus sylvestris, Picea abies, Pinus nigra ssp. laricio, Pinus sylvestris, Populus tremula, Prunus avium, Pseudotsuga menziesii, Quercus cerris, Quercus rubra, Sorbus aria, Sorbus aucuparia, Sorbus torminalis, Taxus baccata, Thuja plicata, Tilia cordata, Tilia platyphyllos, Tilia vulgaris, Tsuga heterophylla, Ulmus glabra) was also collated. A complete list of data sources is provided. Full details about this dataset can be found at https://doi.org/10.5285/22b3d41e-7c35-4c51-9e55-0f47bb845202