This dataset includes measurements made by pressure, temperature, conductivity, backscatter, oxygen, photosynthetically available radiation (PAR) and chlorophyll fluorescence sensors attached to two Seagliders deployed in the Western Ross Sea, Antarctica (-77.420 degrees S and 176.139 degrees E). The gliders produced profiles roughly every 2 hours from the sea surface to the seabed (about 800 m depth) and all observations are un-gridded timeseries. Seaglider 676 (sg676) was deployed on 28 November 2022 and Seaglider 613 (sg613) was deployed on 04 December 2022, both from the fast ice. Both Seagliders were recovered on 18 January 2023 by the R/V Nathaniel B. Palmer (Cruise number NBP23-02). Each glider dataset was processed using the UEA Seaglider toolbox (available at: http://bitbucket.org/bastienqueste/uea-seaglider-toolbox ). The campaign was designed to provide the biophysical conditions to link with the Adelie penguin preyscape. The campaign also sampled the extensive eddy field in the region, and obtained observational data in a key region of very high biological productivity (i.e. the Ross Sea polynya). The Discovery Science project is funded by the Natural Environmental Research Council, UK (NERC) and the National Science Foundation, USA (NSF) (grant reference NE/W00755X/1).