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Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm

In this mesocosm study, three types of wet wipes (plastic containing, and home and commercially compostable) colonised with E. coli were buried in beach sand and their degradation, tensile strength, and concentration of E. coli was quantified over 15 weeks. Wipes were firstly passed through a series of treatments to simulate their journey from the bathroom to the beach. Water characteristics (pH, salinity) of these water types were measured. Full details about this nonGeographicDataset can be found at https://doi.org/10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d

Simple

Date (Publication)
2024-07-15
Identifier
https://catalogue.ceh.ac.uk/id/19a8083b-afa4-40b9-91bf-7a529eea870d
Identifier
doi: / 10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
Other citation details
Metcalf, R., Fellows, R., White, H.L., Quilliam, R. (2024). Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm. NERC EDS Environmental Information Data Centre 10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
Point of contact
  University of Stirling - Metcalf, R.
https://orcid.org/0000-0002-1463-427X
Author
  University of Stirling - Metcalf, R.
https://orcid.org/0000-0002-1463-427X
Author
  University of Stirling - Fellows, R.
Author
  University of Stirling - White, H.L.
Author
  University of Stirling - Quilliam, R.
https://orcid.org/0000-0001-7020-4410
Owner
  University of Stirling
Publisher
  NERC EDS Environmental Information Data Centre
Custodian
  NERC EDS Environmental Information Data Centre
GEMET - INSPIRE themes, version 1.0
  • Human Health and Safety
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Metcalf, R., Fellows, R., White, H.L., Quilliam, R. (2024). Tensile strength and concentrations of E. coli colonising three types of wet wipes in a sand mesocosm. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/19a8083b-afa4-40b9-91bf-7a529eea870d
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Health
  • Environment
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
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Hierarchy level
nonGeographicDataset Non geographic dataset
Other
nonGeographicDataset

Conformance result

Date (Publication)
2010-12-08
Statement
At weekly timepoints (for 15 weeks), four of each wet wipe type were randomly selected, and removed from the mesocosm using sterile forceps. The loosely adhering sand was shaken off and each wet wipe transferred into a sterile glass vial containing 20 ml phosphate-buffered saline (PBS). Vials were then vortexed at 1500 rpm for 5 min and E. coli enumerated by filtering the PBS through 0.45 μm filters, which were placed onto selective media and incubated at 37°C. The wet wipes were removed from the glass vials and left to dry overnight. The following day, a 50 N digital force meter was used to quantify tensile strength by measuring the force required to break each wipe. Wipes were also photographed to determine degradation visually. Background bacterial concentrations were measured by filtering 100 ml water through 0.45 μm cellulose acetate membranes and placed onto selective media.
File identifier
19a8083b-afa4-40b9-91bf-7a529eea870d XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
nonGeographicDataset Non geographic dataset
Hierarchy level name
nonGeographicDataset
Date stamp
2025-03-21T09:38:38
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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Keywords

GEMET - INSPIRE themes, version 1.0
Human Health and Safety

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