Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018
This dataset contains information on the Bacterial Amplicon Sequence Variant (ASV) abundance from peat soil sampled following a wildfire on the Stalybridge estate (UK) in June 2020. Samples were taken in 10 established plots at three time periods following the fire: approximately 3 months, 10 months and 12 months post-fire. This was taken at two depths: 0 – 5cm from the surface (top) and 5 – 10 cm from the surface (bottom). The 10 plots were divided into two groups of five, one that was observed to have suffered a light burn (termed shallow burn) and one that received a more severe burn (termed deep burn). Five additional plots were sampled at the same time as the 3-month samples from a neighbouring unburned site (termed control plots). Samples where no ASVs were observed were removed from the dataset. Full details about this dataset can be found at
https://doi.org/10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad
Simple
- Date (Publication)
- 2022-01-18
- Identifier
- doi: / 10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad
- Other citation details
- Robroek, B.J.M., Shepherd, H.E.R., Sahar, N.E., Dumont, M.G. (2022). Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018. NERC EDS Environmental Information Data Centre 10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad
- GEMET - INSPIRE themes, version 1.0
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- Environmental Monitoring Facilities
- Access constraints
- otherRestrictions Other restrictions
- Other constraints
- no limitations
- Use constraints
- otherRestrictions Other restrictions
- Use constraints
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- If you reuse this data, you should cite: Robroek, B.J.M., Shepherd, H.E.R., Sahar, N.E., Dumont, M.G. (2022). Post-fire microbial data (16S DNA) from burned peat soil on the Stalybridge estate (UK), October 2018. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/01ced3c2-17c6-4512-b73b-e065afed7bad
- Spatial representation type
- textTable Text, table
- Distance
- 10 urn:ogc:def:uom:EPSG::9001
- Metadata language
- EnglishEnglish
- Character set
- utf8 UTF8
- Topic category
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- Biota
- Environment
- Begin date
- 2018-10-01
- End date
- 2018-10-31
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- Unique resource identifier
- GB place names
- Distribution format
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Comma-separated values (CSV)
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Comma-separated values (CSV)
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- Hierarchy level
- dataset Dataset
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- dataset
Conformance result
- Date (Publication)
- 2010-12-08
- Statement
- DNA was extracted from the peat soil using the QIAGEN DNeasy powersoil kit, following standard manual specifications. The extracted DNA was then sequenced through the use of high-throughput sequencing, using 515F/806R primers that target the V4 region of the 16S rRNA. Results from the sequencing were then cleaned and prepared using the DADA2 pipeline to produce the ASV table.
- File identifier
- 01ced3c2-17c6-4512-b73b-e065afed7bad XML
- Metadata language
- EnglishEnglish
- Character set
- ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
- Hierarchy level
- dataset Dataset
- Hierarchy level name
- dataset
- Date stamp
- 2025-11-13T16:21:26
- Metadata standard name
- UK GEMINI
- Metadata standard version
- 2.3
Point of contact
NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg
,
Lancaster
,
LA1 4AP
,
UK
https://eidc.ac.uk/
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NERC Data Catalogue Service