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  • This dataset identified bacteria able to grow in the presence of several Antibiotics in a British agricultural soil, by DNA stable isotope probing (SIP). The dataset was created with samples of the 'heavy' and 'light' fractions of the treatments and also from control soils. The 16S rRNA genes from these samples were amplified and sequenced by barcoded Illumina sequencing. Polymerase chain reaction (PCR) primers 515FB (GTGYCAGCMGCCGCGGTAA) and 806RB (GGACTACNVGGGTWTCTAAT) from the Earth Microbiome project targeting the V4 region of the 16S rRNA gene (approximately 250 nucleotides) were used. Library preparation and sequencing was performed at the National Oceanographic Centre (NOC) of the University of Southampton, UK, following methodologies described by Caporaso et al. (2012). Samples were pooled in an equimolar concentration and sequenced on separate runs for MiSeq using a 2 bp x 300 bp paired end protocol.

  • LCM2007 is a parcel-based thematic classification of satellite image data covering the entire United Kingdom. The map updates and upgrades the Land Cover Map of Great Britain (LCMGB) 1990 and LCM2000. Like the earlier 1990 and 2000 products, LCM2007 is derived from a computer classification of satellite scenes obtained mainly from Landsat, IRS and SPOT sensors. It also covers Northern Ireland and incorporates information derived from other ancillary datasets. LCM2007 was classified using a nomenclature corresponding to the Joint Nature Conservation Committee (JNCC) Broad Habitats, which encompasses the entire range of UK habitats. In addition, it recorded further detail where possible, incorporating land cover classes sought by other users. LCM2007 is produced in both vector and raster formats, with a number of different versions containing varying levels of detail and at different spatial resolutions. This dataset consists of 1km percentages per Target Class. Northern Ireland only.

  • LCM2007 is a parcel-based thematic classification of satellite image data covering the entire United Kingdom. The map updates and upgrades the Land Cover Map of Great Britain (LCMGB) 1990 and LCM2000. Like the earlier 1990 and 2000 products, LCM2007 is derived from a computer classification of satellite scenes obtained mainly from Landsat, IRS and SPOT sensors. It also covers Northern Ireland and incorporates information derived from other ancillary datasets. LCM2007 was classified using a nomenclature corresponding to Joint Nature Conservation Committee (JNCC) Broad Habitats, which encompasses the entire range of UK habitats. In addition, it records further detail where possible, incorporating land cover classes sought by other users. LCM2007 is produced in both vector and raster formats, with a number of different versions containing varying levels of detail and at different spatial resolutions. Product consists of the dominant Target Class per 1km square, raster dataset (Northern Ireland only).

  • This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucleotide Archive (ENA) under accession number PRJEB36347. The dataset contains whole-genome sequencing from 13 natural populations of the Trinidadian guppy (Poecillia reticulata) (n=272) sampled across a range of colour and predation schemes. Data were collected to investigate the ecological genomics of Negative Frequency Dependent Selection (NFDS). Guppy males are colourful, displaying a mosaic of complex and varied colouration patterns; in comparison, females are drab and inconspicuous. Females prefer rare males and rare males show higher reproductive fitness. Rare males also have a significantly higher survival advantage. This project used genome scans to identify the evolutionary signatures of NFDS.

  • This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucleotide Archive (ENA). This dataset contains sequences of the genomic DNA of gut microbiota of calves in response to preventive antibiotic therapy florfenicol obtained by DNA-seq. Importantly, the dataset also contains sequences of genes resistant to different antibiotics. The dataset was created from faecal samples (n=3) of the antibiotic treated animals over seven days and samples (n=3) of animals which have not been subjected to the antibiotic over the same time.

  • This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucleotide Archive (ENA) under accession number PRJEB36506. Guppy (Poecilia reticulata) colour traits have fascinated biologists for a hundred years, and present a unique system for investigating the evolution of colour polymorphism. The aim of this project is to identify regions of the guppy genome responsible for colour patterning in males of the species (the sex which shows colour patterning). Using pool-sequencing, we genotyped four inbred guppy lines (n=48 per line), which originate from a natural guppy population, Paria. Each line was founded by one male showing distinct colouration patterns, and has been backcrossed with unrelated females every generation for ~40 generations, allowing Y-linked colour to be inherited intact from father to son (iso-Y lines).

  • Samples were collected from the slurry tank of a 200-cow dairy farm in the East Midlands once per month between June and October 2017 (n=5). Triplicate extractions were performed on each sample using two extraction kits: PowerFecal Kit (Qiagen) and Isolate II Fecal DNA kit (BioLine) (30 extractions in total). DNA was quantified using a Qubit fluorometer (Invitrogen) while quality was assessed via Nanodrop (ThermoFisher). Extracted DNA was stored at 4˚ C pending sequencing. Metagenomic shotgun sequencing of extracted slurry DNA was performed and demultiplexed by Edinburgh Genomics using the Illumina NovaSeq platform (150bp paired end library). Viral metagenomes were prepared firstly by homogenising cattle slurry in PBS. The homogenate was ultracentrifuged to pellet unwanted solids and bacteria. To further remove bacteria, the supernatant was passed sequentially through 0.45um and 0.22um filters. The filtrate was concentrated on an Amicon column and DNA was extracted using a standard phenol-chloroform extraction. Sequencing was conducted using Illumina Novaseq with a 2x150bp library. This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucletotide Archive.

  • This data is NERC-funded but not held by the EIDC. This data is archived in the National Center for Biotechnology Information (NCBI). This dataset consists of paired-end Illumina MiSeq reads (with primers removed) generated from a 16S rRNA gene metabarcoding study. The majority of the samples processed were faecal samples from a British commercial pig unit (n = 372). Sequences derived from a synthetic mock bacterial community were also included as controls (n = 6)

  • This data is NERC-funded but not held by the EIDC. This data is archived in the European Nucleotide Archive (ENA). This dataset contains sequences of 16S rRNA genes of gut microbiota of calves in response to preventive antibiotic therapy florfenicol by DNA-seq. The dataset was created from faecal samples (n=3) of the antibiotic treated animals over seven days and samples (n=3) of animals which have not been subjected to the antibiotic over the same time.

  • Dataset contains distribution of aerial grass pollen data using environmental metabarcoding. The data containing the rbcL and ITS2 markers for aerial grass pollen across different latitudes across Britain. This data is NERC-funded but not held by the EIDC. This data is archived in NCBI (SRA) https://www.ncbi.nlm.nih.gov/sra/SRP150205