Zooplankton faecal pellet abundance, volume and flux were determined from samples collected at three stations in the Scotia Sea, Southern Ocean during cruise JR304. Samples were collected at six depths within the 0 - 400 m epi- to upper mesopelagic using Niskin bottles attached to a CTD unit and were preserved in a formalin-based solution. Fluorescence data were collected during the same deployments. Sampling was performed by C. Liszka and G. Tarling on board RRS James Clark Ross. Sample analysis was performed by C. Liszka at BAS HQ in Cambridge.