Genetic variation on a spatial scale was assessed, using both DNA fingerprinting and sequencing-based approaches, in the Antarctic endemics Buellia frigida, Carbonia vorticosa and Amandinea petermananii, and in the bipolar species Caloplaca saxicola, Umbilicaria decussata and Cladonia galindezii. PCR-based (Polymerase Chain Reaction) molecular biology techniques, were used as they are ideal for working with lichens because little starting material is required. See Fabian et al. 2007 for further information on analyses and results.
The fieldwork involved collection of fertile lichens from a range of sites across the Antarctic Peninsula and isolation of the lichen-forming fungi into pure culture in a laboratory at Rothera. Approximately 5,600 monospore cultures were isolated, including B frigida. Approximately 400 thalli of Usnea species, and 3 O. frigida thalli have also been collected for whole thallus analysis. Logarithmic sampling transects of B frigida were conducted at Rothera (2 transects) and on Anchorage Island (one transect) to examine the genetic variation and geographic variation. All thalli of B frigida collected from the transects were successfully used to generate viable spores from four individual apothecia from each thallus. 16 spores were subcultured and maintained from each apothecium.
This dataset consists of (i) 673 red-green-blue (RGB) images, (ii) precise coordinates of ground control points and harvest plot corners, (iii) the photogrammetrically reconstructed dense point cloud (comprising of 228,315,000 points with XYZ and RGB values), (iv) four normalised difference vegetation index (NDVI) maps, (v) aboveground biomass data from harvest plots, and (vi) observations of the ground surface obtained from a walkover survey with a GNSS instrument. These data were collected over the eastern part of Qikiqtaruk - Herschel Island, in the Canadian Yukon (69.5N, 138.8W). The images were collected in July and August 2016. Further details on the image processing are provided in the lineage section. This dataset was created by Andrew Cunliffe, with support from Isla Myers-Smith, Jakob Assmann, Jeffery Kerby and Gergana Daskalova (https://teamshrub.com/), in order to inform ongoing ecological monitoring studies in this area. This research was supported by the Natural Environment Research Council (NE/M016323/1), and the NERC Geophysical Equipment Facility (GEF:1063).