ec83a4ea-923a-4f02-8a9f-aeecc43d7123 English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:15:32 UK GEMINI 2.3 WGS 84 E. coli counts of fresh cattle faeces from the North Wyke Farm Platform 2019-09-13 publication https://catalogue.ceh.ac.uk/id/ec83a4ea-923a-4f02-8a9f-aeecc43d7123 10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123 doi: Hodgson, C.J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre 10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123 The data consists of faecal microbiology and moisture content taken from two separate cohorts of 30 cattle. The cattle were from the North Wyke Farm Platform, a UK national capability, located at Rothamsted Research, North Wyke in Devon. Faecal samples were collected between November 2016 and July 2018. Samples were collected and microbiologically analysed in the laboratory within 6 to 8 hours. Two cohorts of 30 cattle were selected from 90 animals, ten from each of the three farmlets. Each cohort covering the period that cattle enter the farm platform, i.e. from weaning until slaughter ca. 16 – 20 months. Full details about this dataset can be found at https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123 Hodgson, C.J. Rothamsted Research chris.hodgson@rothamsted.ac.uk https://orcid.org/0000-0003-4067-3210 ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Hodgson, C. Rothamsted Research chris.hodgson@rothamsted.ac.uk pointOfContact NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian NERC Environmental Information Data Centre info@eidc.ac.uk publisher Environmental Monitoring Facilities theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication E. coli Wikidata 2012-10-29 creation Agriculture otherRestrictions no limitations otherRestrictions © Natural Environment Research Council otherRestrictions This resource is available under the terms of the Open Government Licence otherRestrictions If you reuse this data, you should cite: Hodgson, C.J. (2019). E. coli counts of fresh cattle faeces from the North Wyke Farm Platform. NERC Environmental Information Data Centre https://doi.org/10.5285/ec83a4ea-923a-4f02-8a9f-aeecc43d7123 textTable 5 English utf8 biota farming environment 2016-11-15 2018-07-17 -3.931 -3.877 50.744 50.77 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/ec83a4ea-923a-4f02-8a9f-aeecc43d7123 Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/ec83a4ea-923a-4f02-8a9f-aeecc43d7123.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Faecal samples were taken by members of staff from Rothamsted Research North Wyke. In the laboratory one gram of faecal material was aseptically transferred to nine ml of sterile Ringers (Oxoid, Basingstoke, UK) and appropriate serial 10-fold dilutions were made. An aliquot (0.1 ml) was plated in duplicate on to Membrane Lactose Glucuronide Agar (MLGA) (Oxoid) and spread across the surface of the agar using a disposal plastic spreader (VWR). Antibiotics were added to the MLGA at suitable concentrations after autoclaving and prior to pouring the plates. Antibiotics were added at the following rates; 16 mg/l for tetracycline and cephalexin, 1.0 mg/l marbofloxacin, 8 mg/l meropenem. All plates were allowed to air dry prior to inversion and incubation, at 44.5 ◦C (±0.2 ◦C) for 18–24 h. Colonies were counted, after incubation, and reported as E.coli colony forming units (CFU) per gram fresh weight and also on a dry weight basis. All colony counts were further transformed by log to base 10. Gravimetric moisture content of faecal samples, approximately 50 g of faecal material was accurately weighed into a pre-weighed silver foil container and dried in an oven at 105 ◦C, until constant weight. To calculate % dry matter: 100 – (((fresh wt.-Dry wt.) /(fresh wt.))*100), effectively 100 – moisture content. The establishment licence under which the experiment was conducted was XA11784A2 and the project licence was P592D2677. Data was collected for the NERC funded “Does the potential for AMR selection differ between common UK cattle grazing systems?” NE/N019792/1.