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Data describing pollen identified from honey samples originating from the UKCEH National Honey Monitoring Scheme for 2019

The following data set describes regional and temporal occurrence of plants foraged upon by managed honey bees (Apis mellifera). This data was derived from DNA meta-barcoding of pollen extracted from honey samples provided by bee keepers archived as part of the UK National Honey Monitoring Scheme (https://honey-monitoring.ac.uk/). All data provided is from the first full year of the scheme in 2019. Working in partnership with UK beekeepers, the National Honey Monitoring Scheme aims to use honeybees to monitor long-term changes in the condition and health of the UK countryside. Data associated with subsequent years will be made available as samples are processed. The Honey Monitoring Scheme is supported by national capability funding from UK Centre for Ecology & Hydrology under the ASSIST programme. Full details about this dataset can be found at https://doi.org/10.5285/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5

Simple

Date (Publication)
2022-01-18
Identifier
https://catalogue.ceh.ac.uk/id/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5
Identifier
doi: / 10.5285/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5
Other citation details
Woodcock, B.A., Oliver, A.E., Newbold, L.K., Gweon, H.S., Roy, D.B., Pywell, R.F. (2022). Data describing pollen identified from honey samples originating from the UKCEH National Honey Monitoring Scheme for 2019. NERC EDS Environmental Information Data Centre 10.5285/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5
Author
  UK Centre for Ecology & Hydrology - Woodcock, B.A.
https://orcid.org/0000-0003-0300-9951
Author
  UK Centre for Ecology & Hydrology - Oliver, A.E.
https://orcid.org/0000-0003-4923-277X
Author
  UK Centre for Ecology & Hydrology - Newbold, L.K.
https://orcid.org/0000-0001-8895-1406
Author
  University of Reading - Gweon, H.S.
https://orcid.org/0000-0002-6218-6301
Author
  UK Centre for Ecology & Hydrology - Roy, D.B.
https://orcid.org/0000-0002-5147-0331
Author
  UK Centre for Ecology & Hydrology - Pywell, R.F.
https://orcid.org/0000-0001-6431-9959
Publisher
  NERC EDS Environmental Information Data Centre
Custodian
  NERC EDS Environmental Information Data Centre
Owner
  UK Centre for Ecology & Hydrology
Point of contact
  UK Centre for Ecology & Hydrology - Woodcock, B.A.
https://orcid.org/0000-0003-0300-9951
Maintenance and update frequency
asNeeded As needed
GEMET - INSPIRE themes, version 1.0
  • Environmental Monitoring Facilities
Access constraints
otherRestrictions Other restrictions
Other constraints
no limitations
Use constraints
otherRestrictions Other restrictions
Other constraints
This resource is available under the terms of the Open Government Licence
Use constraints
otherRestrictions Other restrictions
Other constraints
If you reuse this data, you should cite: Woodcock, B.A., Oliver, A.E., Newbold, L.K., Gweon, H.S., Roy, D.B., Pywell, R.F. (2022). Data describing pollen identified from honey samples originating from the UKCEH National Honey Monitoring Scheme for 2019. NERC EDS Environmental Information Data Centre https://doi.org/10.5285/e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5
Spatial representation type
textTable Text, table
Metadata language
EnglishEnglish
Character set
utf8 UTF8
Topic category
  • Biota
Begin date
2019-01-01
End date
2019-12-31
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Reference system identifier
UK regions
Distribution format
  • Comma-separated values (CSV) ()

Distributor
  NERC EDS Environmental Information Data Centre
OnLine resource
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Supporting information

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Hierarchy level
dataset Dataset
Other
dataset

Conformance result

Date (Publication)
2010-12-08
Statement
All honey samples were submitted following an application though the National Honey Monitoring Scheme online portal which required minimum meta-data on site location and sample date which was verified. This included in some cases additional data on hive health metrics. DNA meta-barcoding to rapidly process samples to identify plant species. Quality assurance of the DNA metabarcoding was delivered through operational deployment of sophisticated protocols for barcoding and interpreting large volumes of honey samples. To do this we developed the HONEYPI pipeline implemented in python 2.7 and is open access (https://github.com/hsgweon/honeypi). The HONEYPI pipeline is divided into several parts as follows: 1) the raw amplicon sequences are quality filtered and adapters removed; 2) DADA2 pipeline is subsequently used to generate an Amplicon Sequence Variant (ASV) abundance table containing chimera-removed, high-quality error-corrected sequences. 3). For each ASV, conserved regions flanking ITS2 are removed; and (4) resulting sequences taxonomically classified using the naive Bayesian classifier against in-house ITS2 database. Since HONEYPI uses ASVs rather than clusters of sequences for classification, it allows combining of ASV tables, i.e. data from two or more separate sequencing runs can be merged without re-clustering sequences. A full open access methodological paper describing this approach is given in Oliver et al (2021) MethodsX, 8, 101303 (https://doi.org/10.1016/j.mex.2021.101303)
File identifier
e9ec63be-3f2b-4d1b-b9bf-77ca2b96c7f5 XML
Metadata language
EnglishEnglish
Character set
ISO/IEC 8859-1 (also known as Latin 1) 8859 Part 1
Hierarchy level
dataset Dataset
Hierarchy level name
dataset
Date stamp
2025-11-13T16:26:12
Metadata standard name
UK GEMINI
Metadata standard version
2.3
Point of contact
  NERC EDS Environmental Information Data Centre
Lancaster Environment Centre, Library Avenue, Bailrigg , Lancaster , LA1 4AP , UK
https://eidc.ac.uk/
 
 

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Keywords

GEMET - INSPIRE themes, version 1.0
Environmental Monitoring Facilities

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