e548dc5d-49e3-467d-9435-c199da40e7be English ISO/IEC 8859-1 (also known as Latin 1) dataset dataset NERC EDS Environmental Information Data Centre Lancaster Environment Centre, Library Avenue, Bailrigg Lancaster LA1 4AP UK info@eidc.ac.uk https://eidc.ac.uk/ EIDC website The Environmental Information Data Centre (EIDC) is the UK's national data centre for terrestrial and freshwater sciences. information pointOfContact 2025-11-13T16:17:20 UK GEMINI 2.3 OSGB 1936 / British National Grid Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle 2018-08-10 publication https://catalogue.ceh.ac.uk/id/e548dc5d-49e3-467d-9435-c199da40e7be 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be doi: Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre 10.5285/e548dc5d-49e3-467d-9435-c199da40e7be The data presented are quantitative polymerase chain reaction (qPCR) read outs from antimicrobial resistance gene (AMRG) assays and associated metadata from this project. In this dataset, the mean gene copy numbers per microlitre of DNA extract are shown. The data were collected from faecal and environmental samples which were obtained from a single British commercial pig unit. The former were collected from the sow housing barn, pig growing houses and slurry tanks within the farm unit and the latter were obtained through random stratified sampling of the farm and the surrounding land. These samples were taken from what will be referred to as the 'main study'. A further study was carried out to obtain samples after a partial depopulation which took place on this farm. Faecal samples were obtained from the sow housing barn, pig growing houses and slurry tanks and will be referred to as the 'depopulation (depop) study'. For the main study, the samples were collected between October 19th 2016 and April 5th 2017. For the depop study, the samples were collected between 19th June 2017 and 13th November 2017. The data associated with all samples were generated between August 1st 2017 and May 1st 2018. Full details about this dataset can be found at https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be Pollock, J. The Roslin Institute jolinda.pollock@roslin.ed.ac.uk pointOfContact Pollock, J. SRUC/The Roslin Institute jolinda.pollock@roslin.ed.ac.uk https://orcid.org/0000-0002-2260-592X ORCID record ORCID is an open, non-profit, community-driven effort to create and maintain a registry of unique researcher identifiers and a transparent method of linking research activities and outputs to these identifiers. information author Corbishley, A. R(D)SVS/The Roslin Institute alexander.corbishley@roslin.ed.ac.uk author Hutchings, M.R. SRUC mike.hutchings@sruc.ac.uk author Gally, D.L. The Roslin Institute david.gally@roslin.ed.ac.uk author NERC Environmental Information Data Centre info@eidc.ac.uk publisher NERC EDS Environmental Information Data Centre info@eidc.ac.uk custodian University of Edinburgh EMAIL NOT PROVIDED owner Soil Agricultural and Aquaculture Facilities Human Health and Safety theme GEMET - INSPIRE themes, version 1.0 2008-06-01 publication resistance (biological) livestock farming GEMET - Concepts, version 4.1.3 2019-01-28 creation Agriculture otherRestrictions no limitations otherRestrictions This resource is made available under the terms of the Open Government Licence otherRestrictions © University of Edinburgh otherRestrictions If you reuse this data, you should cite: Pollock, J., Corbishley, A., Hutchings, M.R., Gally, D.L. (2018). Quantification of selected antimicrobial resistance genes in pig faeces on a British commercial pig farm during a typical production cycle. NERC Environmental Information Data Centre https://doi.org/10.5285/e548dc5d-49e3-467d-9435-c199da40e7be textTable English utf8 biota farming 2016-10-19 2017-04-05 -6.628 1.592 52.239 56.4 Comma-separated values (CSV) NERC EDS Environmental Information Data Centre info@eidc.ac.uk distributor https://data-package.ceh.ac.uk/data/e548dc5d-49e3-467d-9435-c199da40e7be Download the data Download a copy of this data download https://data-package.ceh.ac.uk/sd/e548dc5d-49e3-467d-9435-c199da40e7be.zip Supporting information Supporting information available to assist in re-use of this dataset information dataset dataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 Faecal and soil samples were collected from the study farm weekly using spooned universal tubes and were stored at -20 deg C, batch transported on dry ice to the laboratory where they were stored at -80 deg C until DNA extraction was carried out. DNA extracts were quantified and assessed using a NanoDrop spectrophotometer and split into aliquots for storage. DNA extracts were then screened in triplicate by qPCR and the number of gene copies assessed through the use of a standard curve generated by the inclusion of standards with known copy numbers of each target gene. Non-template controls were also included to ensure that non-specific binding or contamination were not occurring. The qPCR machine software (i.e. MxPro) was then used to calculate the number of gene copies in each of the collected samples and the mean was calculated using the three replicates. These values are included in the data set provided.